Nanoparticles guided precise transplantation of varying numbers of mesenchymal stem cells into post–traumatic syrinx in spinal cord injury rat

Zhang C1,2,3,4, Morozova AYu4, Baklaushev VP3, Gubsky IL2, Melnikov PA4, Gabashvily AN6, Wang G1, Li L1, Wu H1, Wang X5, Chekhonin VP2,4
About authors

1 Department of Bone and Soft Tissue Tumors, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin’s Clinical Research Center for Cancer, Tianjin, China

2 Department of Medicinal Nanobiotechnology, Pirogov Russian National Research Medical University, Moscow

3 Federal Medical and Biological Agency, Moscow

4 Department of Basic and Applied Neurobiology, Federal Medical Research Center for Psychiatry and Narcology, Moscow

5 Department of Epidemiology and Biostatistics, First Affiliated Hospital, Army Medical University, Chongqing, China

6 Vavilov Institute of General Genetics Russian Academy of Sciences, Moscow

Correspondеnce should be adressed: Chao Zhang
Huanhu Xi Road, Hexi Area, Tianjin, 300060, China; nc.ude.umt@oahcgnahzrd; gro.noitadnuofoa@sdrahcir.ffoeg

About paper

Funding: the present study was sponsored by China Scholarship Council (201406940004), and Russian Scientific Foundation (16–15–10432).

Received: 2018-08-26 Accepted: 2018-09-25 Published online: 2018-12-31

Spinal cord injury (SCI) is a traumatic injury to the spinal cord which is not a consequence of the disease. Mesenchymal stem cells (MSCs) have gradually become one of the most used stem cells in research and clinic trial. Based on the previous reports employed the cells ranged from 4 • 105 to 1 • 106, the present study was performed to figure out the best number of MSCs for transplantation of the chronic SCI. Magnetic nanoparticles were used for proving the precise transplantation strategy. Using magnetic resonance imaging (MRI), diffusion tensor imaging (DTI), diffusion tensor tractography (DTT), and behavior testing evaluations, we focused the effect of varying numbers of MSCs on reducing lesion cavity and post–traumatic syrinx formation, suppressing glial scar formation, enhancing neuronal fibers remodeling, promoting axonal regeneration and sprouting, improving vascularization, ameliorating the neuronal factors expressional level, and function improvement. Magnetic nanoparticles were precisely transplanted into the post–traumatic syrinx (PTS). MSCs can restore function after chronic SCI through stimulating the regeneration and sprouting of the axons, reducing the formation of PTS. The effect of MSCs on PTS management and functional improvement post chronic SCI was cell number–dependent, and within the range of 4 • 105 to 1 • 106, 1 • 106 cells were proved to be the best dose.

Keywords: nanoparticles, mesenchymal stem cells, spinal cord injury, cell transplantation