ORIGINAL RESEARCH

Combination of ribosome and phage display for fast selection of high affinity VHH antibody fragments

About authors

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia

Correspondence should be addressed: Stepan P. Chumakov
Miklukho-Maklaya, 16/10, Moscow, 117997; moc.liamg@lukhtah

About paper

Funding: the work was funded by MESR, project code RFMEFI60716X0156.

Author contribution: Kravchenko YuE — Alpaca blood collection and processing, RNA extraction, ELISA; Ivanov SV — construction of libraries of VHH-fragments of antibodies, selection by phage display; Kravchenko DS — protein purification, experiments with eucaryotic cell cultures; Frolova EI — research planning, animal immunization, manuscript editing; Chumakov SP — research planning, selection by ribosome display, analysis of the results, writing a manuscript.

Received: 2018-12-08 Accepted: 2018-12-22 Published online: 2019-02-25
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Fig. 1. Schematic representation of selection process combining ribosome and phage display
Fig. 2. Enrichment of libraries of VHH fragments during selection: with ribosome display (A); with phage display (B); with combination of ribosome and phage display (C). Comparison of enrichment by all methods for VHH3 library (D)
Fig. 3. Comparison of binding properties of five selected VHH fragments in ELISA
Table 1. Primer sequences used for amplification of VHH fragments