ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Combination of ribosome and phage display for fast selection of high affinity VHH antibody fragments
About authors
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia
Correspondence should be addressed: Stepan P. Chumakov
Miklukho-Maklaya, 16/10, Moscow, 117997;
moc.liamg@lukhtah
About paper
Funding: the work was funded by MESR, project code RFMEFI60716X0156.
Author contribution: Kravchenko YuE — Alpaca blood collection and processing, RNA extraction, ELISA; Ivanov SV — construction of libraries of VHH-fragments of antibodies, selection by phage display; Kravchenko DS — protein purification, experiments with eucaryotic cell cultures; Frolova EI — research planning, animal immunization, manuscript editing; Chumakov SP — research planning, selection by ribosome display, analysis of the results, writing a manuscript.
Received: 2018-12-08
Accepted: 2018-12-22
Published online: 2019-02-25
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Fig. 1. Schematic representation of selection process combining ribosome and phage display
Fig. 2. Enrichment of libraries of VHH fragments during selection: with ribosome display (A); with phage display (B); with combination of ribosome and phage display (C). Comparison of enrichment by all methods for VHH3 library (D)
Fig. 3. Comparison of binding properties of five selected VHH fragments in ELISA
Table 1. Primer sequences used for amplification of VHH fragments
