2019 / 01

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DOI: 10.24075/brsmu.2019.002

ORIGINAL RESEARCH

Combination of ribosome and phage display for fast selection of high affinity VHH antibody fragments

Kravchenko YuE, Ivanov SV, Kravchenko DS, Frolova EI, Chumakov SP
About authors

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia

Correspondence should be addressed: Stepan P. Chumakov
Miklukho-Maklaya, 16/10, Moscow, 117997; moc.liamg@lukhtah

About paper

Funding: the work was funded by MESR, project code RFMEFI60716X0156.

Author contribution: Kravchenko YuE — Alpaca blood collection and processing, RNA extraction, ELISA; Ivanov SV — construction of libraries of VHH-fragments of antibodies, selection by phage display; Kravchenko DS — protein purification, experiments with eucaryotic cell cultures; Frolova EI — research planning, animal immunization, manuscript editing; Chumakov SP — research planning, selection by ribosome display, analysis of the results, writing a manuscript.

Received: 2018-12-08 Accepted: 2018-12-22 Published online: 2019-02-25
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