ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2019 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Evaluation of the ejaculate microbiota by real-time PCR and culture-based technique
About authors
1 Ural State Medical University, Yekaterinburg, Russia
2 Medical Center “Garmonia”, Yekaterinburg, Russia
Correspondence should be addressed: Еkaterina S. Voroshilina
Furmanova 30, Yekaterinburg, 620142; moc.liamg@anilihsorov
About paper
Acknowledgments: the authors would like to thank V. N. Khayutin, director of Garmonia Medical Center, for allowing to conduct the study in the clinic's laboratory department.
Author contribution: Voroshilina ES — organization of the study, conducting tests, data analysis, article authoring; Zornikov DL — data analysis, statistical processing, article authoring; Panacheva EA — literature review, clinical data collection, statistical analysis, article authoring.
Received: 2019-02-11
Accepted: 2019-03-28
Published online: 2019-03-11
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Table 1. Semen microbiota variants, RT PCR data (n = 86)
Note: 1 — this microbiota type was suggested due to the specifics of the Androflor test (Enterobacteriaceae spp. / Enterococcus spp. are detected in a tube separately from other Gram-positive and Gram-negative facultative anaerobes without species identification); 2 — this variant was applied when a proportion of several detected groups of microorganisms slightly differed from each other; 3 — Fisher's test enabled calculation of significance of the differences.
Table 2. Comparison of the predominant groups of microorganisms according to culture-based technique and RT PCR results (n = 86)
