Copyright: © 2019 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).

ORIGINAL RESEARCH

Evaluation of the ejaculate microbiota by real-time PCR and culture-based technique

About authors

1 Ural State Medical University, Yekaterinburg, Russia

2 Medical Center “Garmonia”, Yekaterinburg, Russia

Correspondence should be addressed: Еkaterina S. Voroshilina
Furmanova 30, Yekaterinburg, 620142; moc.liamg@anilihsorov

About paper

Acknowledgments: the authors would like to thank V. N. Khayutin, director of Garmonia Medical Center, for allowing to conduct the study in the clinic's laboratory department.

Author contribution: Voroshilina ES — organization of the study, conducting tests, data analysis, article authoring; Zornikov DL — data analysis, statistical processing, article authoring; Panacheva EA — literature review, clinical data collection, statistical analysis, article authoring.

Received: 2019-02-11 Accepted: 2019-03-28 Published online: 2019-03-11
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  1. Cornelia Gottschick, Zhi-Luo Deng, Marius Vital et al. The urinary microbiota of men and women and its changes in women during bacterial vaginosis and antibiotic treatment. Microbiome. 2017; 5 (99): 1–15.
  2. Hou D, Zhou X, Zhong X, et al. Microbiota of the seminal fluid from healthy and infertile men. FertilSteril. 2013 Nov; 100(5): 1261–9.
  3. Nelson DE, Dong Q, Van der Pol B, et al. Bacterial communities of the coronal sulcus and distal urethra of adolescent males. PLoS One. 2012; 7 (5): e36298.
  4. Weng SL, Chiu CM, Lin FM, et al. Bacterial communities in semen from men of infertile couples: metagenomic sequencing reveals relationships of seminal microbiota to semen quality. PLoS One. 2014 Oct 23; 9 (10): e110152.
  5. Domes T, Lo KC, Grober ED, et al. The incidence and effect of bacteriospermia and elevated seminal leukocytes on semen parameters. Fertil Steril. 2012 May; 97 (5): 1050–5.
  6. Mändar R, Punab M, Korrovits P, et al. Seminal microbiome in men with and without prostatitis. Int J Urol. 2017 Mar; 24 (3): 211–6.
  7. Schuppe H-C, Pilatz A, Hossain H, et al. Urogenital Infection as a Risk Factor for Male Infertility. Deutsches Aerzteblatt Online. 2017. DOI: 10.3238/arztebl.2017.0339.
  8. Bozhedomov V. A. Hronicheskij prostatit: novaja paradigma lechenija. Urologija. 2016; (36): 78–90. Russian.
  9. Instrukcija po primeneniju nabora reagentov dlja issledovanija mikroflory urogenital'nogo trakta muzhchin metodom PCR v rezhime real'nogo vremeni Androflor® Skrin (OOO NPO «DNK-Tehnologija»). Dostupno po ssylke: http:www.dna-technology.ru/ information/aboutamethod/. Russian.
  10. Jungwirth A, Diemer T, Kopa Z, et al. EAU Guidelines on Male Infertility. 2017. Available from: https://www.researchgate.net/publication/318239925_EAU_Guidelines_on_Male_Infertility_2017.
  11. Aragón IM, Herrera-Imbroda B, Queipo-Ortuño MI, et al. The Urinary Tract Microbiome in Health and Disease. Eur Urol Focus. 2018 Jan; 4 (1): 128–38.