ORIGINAL RESEARCH
Evaluation of the ejaculate microbiota by real-time PCR and culture-based technique
1 Ural State Medical University, Yekaterinburg, Russia
2 Medical Center “Garmonia”, Yekaterinburg, Russia
Correspondence should be addressed: Еkaterina S. Voroshilina
Furmanova 30, Yekaterinburg, 620142; moc.liamg@anilihsorov
Acknowledgments: the authors would like to thank V. N. Khayutin, director of Garmonia Medical Center, for allowing to conduct the study in the clinic's laboratory department.
Author contribution: Voroshilina ES — organization of the study, conducting tests, data analysis, article authoring; Zornikov DL — data analysis, statistical processing, article authoring; Panacheva EA — literature review, clinical data collection, statistical analysis, article authoring.
- Cornelia Gottschick, Zhi-Luo Deng, Marius Vital et al. The urinary microbiota of men and women and its changes in women during bacterial vaginosis and antibiotic treatment. Microbiome. 2017; 5 (99): 1–15.
- Hou D, Zhou X, Zhong X, et al. Microbiota of the seminal fluid from healthy and infertile men. FertilSteril. 2013 Nov; 100(5): 1261–9.
- Nelson DE, Dong Q, Van der Pol B, et al. Bacterial communities of the coronal sulcus and distal urethra of adolescent males. PLoS One. 2012; 7 (5): e36298.
- Weng SL, Chiu CM, Lin FM, et al. Bacterial communities in semen from men of infertile couples: metagenomic sequencing reveals relationships of seminal microbiota to semen quality. PLoS One. 2014 Oct 23; 9 (10): e110152.
- Domes T, Lo KC, Grober ED, et al. The incidence and effect of bacteriospermia and elevated seminal leukocytes on semen parameters. Fertil Steril. 2012 May; 97 (5): 1050–5.
- Mändar R, Punab M, Korrovits P, et al. Seminal microbiome in men with and without prostatitis. Int J Urol. 2017 Mar; 24 (3): 211–6.
- Schuppe H-C, Pilatz A, Hossain H, et al. Urogenital Infection as a Risk Factor for Male Infertility. Deutsches Aerzteblatt Online. 2017. DOI: 10.3238/arztebl.2017.0339.
- Bozhedomov V. A. Hronicheskij prostatit: novaja paradigma lechenija. Urologija. 2016; (36): 78–90. Russian.
- Instrukcija po primeneniju nabora reagentov dlja issledovanija mikroflory urogenital'nogo trakta muzhchin metodom PCR v rezhime real'nogo vremeni Androflor® Skrin (OOO NPO «DNK-Tehnologija»). Dostupno po ssylke: http:www.dna-technology.ru/ information/aboutamethod/. Russian.
- Jungwirth A, Diemer T, Kopa Z, et al. EAU Guidelines on Male Infertility. 2017. Available from: https://www.researchgate.net/publication/318239925_EAU_Guidelines_on_Male_Infertility_2017.
- Aragón IM, Herrera-Imbroda B, Queipo-Ortuño MI, et al. The Urinary Tract Microbiome in Health and Disease. Eur Urol Focus. 2018 Jan; 4 (1): 128–38.