ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2019 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Methods for DNA quantification yield similar relative but different absolute values
About authors
1 Vavilov Institute of General Genetics, Moscow, Russia
2 Research Center for Medical Genetics, Moscow, Russia
3 Biobank of North Eurasia, Moscow, Russia
Correspondence should be addressed: Oleg P. Balanovsky
Gubkina 3, Moscow, 119991; ur.xobni@yksvonalab
About paper
Funding: this study was part of the Government contract with the Research Center for Medical Genetics (Experiment 1). Experiment 2 was supported in part by Grant 17-14-01345 of the Russian Science Foundation.
Author contribution: Balanovsky OP conceived the study, analyzed the obtained data and wrote this manuscript; Kagazezheva ZhA conducted one of the experiments; Olkova MV conducted one of the experiments and analyzed the literature.
Received: 2019-06-21
Accepted: 2019-06-27
Published online: 2019-06-30
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Fig. 1. DNA concentrations in individual samples measured using different methods. A: Experiment 1. B Experiment 2. The X-axis: concentrations yielded by
Quantifiler. The Y-axis: concentrations yielded by Quantifiler (the black line), Qubit (the blue line) and NanoDrop (the red line).
Table 1. Results of DNA quantification in individual samples (Experiment 1)
Table 2. Results of DNA quantification in individual samples (Experiment 2)
Table 3. Correlation coefficients for DNA concentrations yielded by different quantification methods. Above diagonal: experiment 1. Below diagonal: experiment 2
Table 4. Mean DNA concentrations yielded by different quantification methods
