2019 / 03

Next Paper
DOI: 10.24075/brsmu.2019.043

ORIGINAL RESEARCH

Methods for DNA quantification yield similar relative but different absolute values

Balanovsky OP1,2,3, Kagazezheva ZhA1,2, Olkova MV2
About authors

1 Vavilov Institute of General Genetics, Moscow, Russia

2 Research Center for Medical Genetics, Moscow, Russia

3 Biobank of North Eurasia, Moscow, Russia

Correspondence should be addressed: Oleg P. Balanovsky
Gubkina 3, Moscow, 119991; ur.xobni@yksvonalab

About paper

Funding: this study was part of the Government contract with the Research Center for Medical Genetics (Experiment 1). Experiment 2 was supported in part by Grant 17-14-01345 of the Russian Science Foundation.

Author contribution: Balanovsky OP conceived the study, analyzed the obtained data and wrote this manuscript; Kagazezheva ZhA conducted one of the experiments; Olkova MV conducted one of the experiments and analyzed the literature.

Received: 2019-06-21 Accepted: 2019-06-27 Published online: 2019-06-30
|
  1. Bhat S, Curach N, Mostyn T, Bains GS, Griffiths KR, et al. Comparison of methods for accurate quantification of DNA mass concentration with traceability to the international system of units. Anal Chem. 2010 Sep 1; 82 (17): 7185–92. DOI: 10.1021/ ac100845m.
  2. Li X, Wu Y, Zhang L, Cao Y, Li Y, Li J, et al. Comparison of three common DNA concentration measurement methods. Anal Biochem. 2014 Apr 15; (451): 18–24. DOI: 10.1016/j. ab.2014.01.016.
  3. Nielsen K, Mogensen HS, Hedman J, Niederstätter H, Parson W, Morling N. Comparison of five DNA quantification methods. Forensic Science International: Genetics. 2008; 2 (3): 226–30.
  4. He HJ, Stein EV, DeRose P, Cole KD. Limitations of methods for measuring the concentration of human genomic DNA and oligonucleotide samples. Biotechniques. 2018 Feb 1; 64 (2): 59–68. DOI: 10.2144/btn-2017-0102.
  5. Nakayama Y, Yamaguchi H, Einaga N, Esumi M. Pitfalls of DNA Quantification Using DNA Binding Fluorescent Dyes and Suggested Solutions. PLoS ONE. 2016; 11 (3): e0150528. DOI 10.1371/journal.pone.0150528.
  6. Belenikin MS, Galahova AA, Balanovskaya EV, Balanovsky OP. Ocenka vosproizvodimosti fluometricheskogo izmereniya koncentracii DNK. Genetika. 2018; (54): 113–6. DOI: 10.1134/ S0016675818130040. Russian.
  7. Balanovskaya EV, Zhabagin MK, Agdzhoyan AT, Chukhryaeva MI, Markina NV, Balaganskaya OA, et al. Population biobanks: Organizational models and prospects of application in gene geography and personalized medicine. Russian Journal of Genetics. 2016; 52 (12): 1227–43.
  8. Qubit dsDNA BR Assay Kits Manual (MAN0002325, Revision: A.0). Available from: https://tools.thermofisher.com/content/sfs/ manuals/Qubit_dsDNA_BR_Assay_UG.pdf.