2019 / 04

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DOI: 10.24075/brsmu.2019.048

ORIGINAL RESEARCH

Application of culture-based, mass spectrometry and molecular methods to the study of gut microbiota in children

Efimov BA, Chaplin AV, Sokolova SR, Chernaia ZA, Pikina AP, Savilova AM, Kafarskaya LI
About authors

Pirogov Russian National Research Medical University, Moscow, Russia

Correspondence should be addressed: Boris A. Efimov
Ostrovityanova St. 1, Moscow, 117997; ur.liam@ab_vomife

About paper

Funding: the study was supported by Russian Science Foundation (research grant № 17-15-01488).

Author contribution: Efimov BA — research planning, literature analysis, screening children, specimen collection, microbiological research, mass spectrometry research, analysis and interpretation of data, preparing a draft manuscript; Chaplin AV — research planning, literature analysis, isolation of bacterial DNA, carrying out PCR, amplicons purification for sequencing, data analysis and interpretation, preparing a draft manuscript; Sokolova SR — research planning, literature analysis, specimen collection, microbiological research, isolation of bacterial DNA, carrying out PCR, amplicons purification for sequencing, data analysis and interpretation preparing a draft manuscript; Chernaia ZA — research planning, literature analysis, microbiological research, mass spectrometry research, data analysis and interpretation, preparing a draft manuscript; Pikina AP — research planning, literature analysis, microbiological research, mass spectrometry research, data analysis and interpretation, preparing a draft manuscript; Savilova AM — literature analysis, microbiological research, preparing a draft manuscript; Kafarskaya LI — research planning, literature analysis, screening children, microbiological research, analysis and interpretation of data, preparing a draft manuscript

Received: 2019-06-27 Accepted: 2019-07-12 Published online: 2019-08-09
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