ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Comparative phylogenetic analysis of Neisseria gonorrhoeae clinical isolates in Russia, European Union, and Japan
About authors
1 Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia
2 State Research Center of Dermatovenerology and Cosmetology, Russian Ministry of Health, Moscow, Russia
Correspondence should be addressed: Boris L. Shaskolskiy
Vavilova, 32, Moscow, 119991;
ur.pihcoib@yiksloksahs.b
About paper
Funding: the study was supported by the Russian Science Foundation (Project 17-75-20039 on the assessment of genetic diversity of sequence types) and the Ministry of Science and Higher Education of the Russian Federation (Agreement № 075-15-2019-1660 on the collection and verification of clinical isolates and the establishment of the association between the unique sequence types and the population size). The isolates were sequenced at the facilities of the Genome center for collective use (Engelhardt Institute of Molecular Biology; http://www.eimb.ru/ru1/ckp/ccu_genome_c.php).
Compliance with ethical standards: the study was approved by the Ethics Committee of the State Research Centre of Dermatovenerology and Cosmetology (Protocol № 11, dated November 29, 2019). Specimens were collected in compliance with the Declaration of Helsinki (2000) and the European Convention on Human Rights and Biomedicine (1999).
Author contribution: Shaskolskiy BL, Dementieva EI, Kandinov ID carried out the study, analyzed the data and wrote the manuscript; Gryadunov DA supervised the study and wrote the manuscript; Chestkov AV, Solomka VS, Kubanov AA, Deryabin DG collected and verified clinical isolates, analyzed the obtained data.
Received: 2020-01-09
Accepted: 2020-02-08
Published online: 2020-02-22
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Fig. 1. A phylogenetic tree of N. gonorrhoeae NG-MAST types detected in Russia and EU. Branches with bootstrap values of 80-89 are shown in blue; red indicates
bootstrap value of 90-100. Phylogenetic clades (bootstrap values ≥ 90%) are designated by Greek letters α–π. Black arrows indicate NG-MAST types that are most
common in Russia. Gray dashed arrows indicate NG-MAST types that are most common in European countries
Fig. 2. Associations between the proportion of N. gonorrhoeae isolates of unique sequence types and the country population size. Country codes are given in Table 1. А. A dendrogram. The clusters are indicated by numbers. B. The relationship between the proportion of N. gonorrhoeae clinical isolates representing unique sequence types and the population size
Table 1. The most common NG-MAST types found in the Russian population (2013–2018)
Note: * — NG-MAST types unique to Russia
Table 2. NG-MAST types most common to EU and Japan
Note: * — NG-MAST types unique to the specified country; ** — NG-MAST types common to Poland and described in [17].
Table 3. Distribution of isolates of unique NG-MAST types (2013)
