2020 / 02

DOI: 10.24075/brsmu.2020.026

OPINION

Strategies of RT-PCR-based assay design and surveillance of SARS-CoV-2

Kuznetsova NA1, Pochtovyy AA1,2, Nikiforova MA1, Gushchin VA1,2
About authors

1 N.F. Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia

2 Lomonosov Moscow State University, Moscow, Russia

Correspondence should be addressed: Nadezhda A. Kuznetsova
Gamalei, 16, str. 1, Moscow, 123098; moc.liamg@0avostenzukaydan

About paper

Author contribution: the autors equally contributed to the manuscript.

Received: 2020-04-21 Accepted: 2020-04-28 Published online: 2020-04-30
|
  1. Li Q, et al. Early transmission dynamics in Wuhan, China, of novel coronavirus-infected pneumonia. N Engl J Med. 2020; 382: 1199–207.
  2. Available from: https://gisanddata.maps.arcgis.com/apps/ opsdashboard/index.html#/bda7594740fd40299423467b48e9ecf6.
  3. Kissler SM, Tedijanto C, Goldstein E, Grad YH, Lipsitch M. Projecting the transmission dynamics of SARS-CoV-2 through the postpandemic period. Science. 2020; eabb5793.
  4. He X, Lau EHY, Wu P, et al. Temporal dynamics in viral shedding and transmissibility of COVID-19. Nat Med. 2020. Available from: https://doi.org/10.1038/s41591-020-0869-5.
  5. Cheng MP, Papenburg J, Desjardins M, Kanjilal S, Quach C, Libman M, et al. Diagnostic Testing for Severe Acute Respiratory Syndrome–Related Coronavirus-2: A Narrative Review. Ann Intern Med. 2020; Apr 13: [Epub ahead of print]. DOI: 10.7326/M20-1301.
  6. Shen M, Zhou Y, Ye J, Abdullah Al-Maskri AA, Kang Y, Zeng S, et al. Recent advances and perspectives of nucleic acid detection for coronavirus. J Pharm Anal. 2020 Mar 1. Available from: https:// www.journals.elsevier.com/journal-of-pharmaceutical-analysis.
  7. Wang W, Xu Y, Gao R, Lu R, Han K, Wu G, et al. Detection of SARS-CoV-2 in Different Types of Clinical Specimens. JAMA. 2020 Mar 11.
  8. Pan Y, Zhang D, Yang P, Poon LLM, Wang Q.Viral load of SARS-CoV-2 in clinical samples. Lancet Infect Dis. 2020 Apr; 20 (4): 411–2.
  9. Zou L, Ruan F, Huang M, Liang L, Huang H, Hong Z, et al. SARS-CoV-2 Viral Load in Upper Respiratory Specimens of Infected Patients. N Engl J Med. 2020 Mar 19; 382 (12): 1177–9.
  10. Marty FM, Chen K, Verrill KA. How to Obtain a Nasopharyngeal Swab Specimen April 17, 2020. DOI: 10.1056/NEJMvcm2010260.
  11. Cheng MP, Papenburg J, Desjardins M, Kanjilal S, Quach C, Libman M, et al. Diagnostic Testing for Severe Acute Respiratory Syndrome–Related Coronavirus-2: A Narrative Review. Ann Intern Med. 2020 Apr; 13.
  12. Pang J, Wang MX, Ang IYH, Tan SHX, Lewis RF, et al. Potential Rapid Diagnostics, Vaccine and Therapeutics for 2019 Novel Coronavirus (2019-nCoV): A Systematic Review. J Clin Med. 2020 Feb 26; 9 (3): pii E623.
  13. Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020 Jan; 25 (3).
  14. Available from: https://www.who.int/docs/default-source/ coronaviruse/peiris-protocol-16-1-20.pdf?sfvrsn=af1aac73_4.
  15. R Lu, X Wu, Z Wan, Y Li, L Zuo, J Qin, et al. Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2. Virologica Sinica. 2020; с .1.
  16. Available from: https://www.thermofisher.com/ru/ru/home/life-science/sequencing/dna-sequencing/microbial-sequencing/ microbial-identification-ion-torrent-next-generation-sequencing/ viral-typing/coronavirus-research.html.
  17. Li C, et al. High sensitivity detection of coronavirus SARS-CoV-2 using multiplex PCR and a multiplex-PCR-based metagenomic method. bioRxiv. 2020.
  18. Available from: https://nanoporetech.com/about-us/news/artic-network-provides-protocol-rapid-accurate-sequencing-novel-coronavirus-ncov-2019.