Interleukin dynamics during cognitive stress in patients with chronic cerebral ischemia

About authors

Research Center of Neurology, Moscow, Russia

Correspondence should be addressed: Vitaly Fedorovich Fokin
Volokolamskoe shosse, 80, Moscow, 125367; ur.liam@fvf

About paper

Author contribution: Fokin VF performed data analysis and wrote the manuscript; Shabalina AA performed biochemical analysis of cytokines and participated in writing the manuscript; Ponomareva NV collected and analyzed psychometric data, participated in writing the manuscript; Medvedev RB performed clinical examinations, analyzed the literature and proposed the study design; Lagoda OV analyzed clinical data and proposed the study design; Tanashyan MM proposed the study design, summarized clinical data in the context of the obtained results.

Compliance with ethical standards: the study was approved by the Ethics Committee of the Research Center of Neurology (Protocol No. 11/14 dated November 19, 2014); all study participants signed informed consent to participate.

Received: 2020-11-06 Accepted: 2020-12-17 Published online: 2020-12-28

Neuroimmune interactions represent a highly dynamic mechanism for the regulation of cognitive function in chronic cerebral ischemia (CCI). The aim of this study was to investigate changes in salivary proinflammatory cytokines IL1β and IL6 and anti-inflammatory IL10 in patients with CCI (mean age 65.4 ± 9.1 years) before and after cognitive tests. After cognitive tests, the levels of salivary IL1β and IL6 were significantly elevated by 101.6 ± 19.1 pg/ml (n = 74) and 32.8 ± 6.1 pg/ml (n = 74), respectively. Using one-way ANOVA ana non-parametric statistical methods, we were able to demonstrate associations between changes in salivary interleukins and cognitive performance. In the group of patients with a significant increase in IL1β, some cognitive parameters were lower than in the group with negative or zero dynamics of this cytokine: the patients made more mistakes in the subtraction test (F = 11.5; n = 63; p = 0.001) and performed worse in the Luria test (F = 6.8; n = 65; p = 0.01). For IL6, Spearman’s rank correlation coefficient for the number of mistakes in the subtraction test was positive and differed significantly from 0 (R = 0.26; n = 62; p = 0.042). The group with positive IL10 dynamics performed better in N-back test (F = 5.2; n = 67; p = 0.03) and made fewer mistakes in the subtraction test (F = 6.8; n = 63; p = 0.01) in comparison with patients who demonstrated negative IL10 dynamics. Good performance in other cognitive tests was not correlated with interleukin dynamics. The article also discusses possible mechanisms underlying interleukin effects on cognitive function in patients with CCI and applications of the obtained data.

Keywords: interleukins, IL10, cognitive function, neuroimmune interactions, vascular encephalopathy, IL1β, IL6