2021 / 03

DOI: 10.24075/brsmu.2021.032
Copyright: © 2021 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).

ORIGINAL RESEARCH

Changes in amino acid profile of cord blood plasma and amniotic fluid of mothers with COVID-19

Lomova NA, Chagovets VV, Dolgopolova EL, Novoselova AV, Petrova UL, Shmakov RG, Frankevich VE
About authors

Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia

Correspondence should be addressed: Natalia A. Lomova
Oparina, 4, Moscow, 117997; ur.xednay@avomol-ahsatan

About paper

Funding: the study was supported by RFBR grant №. 20-04-60093.

Author contribution: Lomova NA — analysis of clinical data, systematic analysis, manuscript writing; Chagovets VV — mass spectrometry-based metabolome analysis, statistical analysis of the results, manuscript editing; Dolgopolova EL — collection and preparation of biological matrix samples in the red zone, statistical analysis of the results; Novoselova AV — mass spectrometry-based metabolome analysis, mass spectrometry data processing; Petrova UL — collection and preparation of biological matrix samples in the red zone; Shmakov RG — analysis of clinical data in the red zone, systematic analysis, manuscript editing; Frankevich VE — preparation of the study, systematic analysis, manuscript writing and editing.

Compliance with ethical standards: the study was approved by the Ethics Committee of the National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V. I. Kulakov (protocol № 13 dated December 10, 2020); the study met the requirements of the Declaration of Helsinki, International Conference on Harmonization (ICF), Good Clinical Practice (GCP), and Federal Law № 323-FZ “On the Basics of Protecting Citizens' Health in the Russian Federation” of November 21, 2011; the informed consent was submitted by all patients.

Received: 2021-05-24 Accepted: 2021-06-18 Published online: 2021-06-29
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Fig. 1. Amino acid concentrations in amniotic fluid of controls and COVID-19 patients. The first and the third quartiles form the boundaries of the box, the median is shown as a line in the center of the box; the ends of whiskers represent the following: one and a half times interquartile range subtracted from the first quartile, sum of the third quartile and one and a half times interquartile range; * — р ≤ 0.05; ** — р ≤ 0.01; *** — р ≤ 0.001; 1-mHis — 1-methylhistidine; 3-mHis — 3-methylhistidine; BAIBA — 3-aminoisobutyric acid; 5-OH-Lys — 5-hydroxylysine; MEA — ethanolamine; ABA — 2-aminobutyric acid; AAD — 2-aminoadipic acid; Car — carnosine; Cit — citrulline; Cyt — cystathionine; 4-OH-Pro — 4-hydroxyproline
Fig. 2. ROC curves for logistic regression models constructed for classification of controls and COVID-19 patients based on amino acid concentrations in amniotic fluid
Fig. 3. Amino acid concentrations in cord blood plasma of controls and COVID-19 patients. The first and the third quartiles form the boundaries of the box, the median is shown as a line in the center of the box; the ends of whiskers represent the following: one and a half times interquartile range subtracted from the first quartile, sum of the third quartile and one and a half times interquartile range; * — р ≤ 0.05; ** — р ≤ 0.01; *** — р ≤ 0.001. 1-mHis — 1-methylhistidine; 3-mHis – 3-methylhistidine; bAla — beta-alanine; BAIBA — 3-aminoisobutyric acid; 5-OH-Lys – 5-hydroxylysine; MEA – ethanolamine; ABA — 2-aminobutyric acid; AAD —2-aminoadipic acid; Car — carnosine; Cit — citrulline; Cyt — cystathionine; 4-OH-Pro — 4-hydroxyproline
Fig. 4. ROC curves for logistic regression models constructed for classification of controls and COVID-19 patients based on amino acid concentrations in cord blood plasma
Table 1. Clinical characteristics of pregnant women with COVID-19
Table 2. Clinical characteristics of pregnant women enrolled
Table 3. Amino acid concentrations (nmol/mL) in amniotic fluid of controls and COVID-19 patients
Table 4. Parameters of logistic regression models allowing one to distinguish patients with COVID-19 from patients with no COVID-19 based on the amino acid concentrations in the amniotic fluid
Table 5. Characteristics of logistic regression models allowing one to distinguish patients with COVID-19 from patients with no COVID-19 based on the amino acid concentrations in the amniotic fluid
Table 6. Amino acid concentrations (nmol/mL) in cord blood plasma of controls and COVID-19 patients
Table 7. Parameters of logistic regression models allowing one to distinguish patients with COVID-19 from patients with no COVID-19 based on the amino acid concentrations in the cord blood plasma
Table 8. Characteristics of logistic regression models allowing one to distinguish patients with COVID-19 from patients with no COVID-19 based on the amino acid concentrations in the blood cord plasma
Table 9. Amino acid concentrations (nmol/mL) in amniotic fluid and cord blood plasma showing significant differences between controls and COVID-19 patients