ISSN Print 2500–1094
ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Features of the pathogenetic mechanisms of tuberculous peritonitis in an experiment
About authors
1 Moscow Research and Clinical Center for TB Control, Moscow, Russia
2 Pirogov Russian National Research Medical University, Moscow, Russia
3 Saint-Petersburg State Research Institute of Phtisiopulmonology, St. Petersburg, Russia
Correspondence should be addressed: Dmitry Vladimirovich Plotkin
Ostrovityanova, 1, Moscow, 117997; ur.tsil@31nk
About paper
Author contributions: Plotkin DV, Vinogradova TI, Sinitsyn MV, Bogorodskaya EM — study concept and design development; Plotkin DV, Reshetnikov MN, Zhuravlev VYu, Zyuzya YuR — material collection; Reshetnikov MN, Zhuravlev VYu — statistical processing; Plotkin DV, Vinogradova TI, Ariel BM, Yablonsky PK — analysis of the data obtained; Plotkin DV, Ariel BM, Sinitsyn MV, Bogorodskaya EM — text preparation; Vinogradova TI, Zyuzya YuR, Yablonsky PK — editing.
Compliance with ethical standards: the study was approved by the Ethics Committee of the Saint Petersburg Research Institute of Phthisiopulmonology (protocol № 73 of December 23, 2020). All manipulations with animals conformed to the requirements of the European Convention for the Protection of Vertebral Animals Used for Experimental and Other Scientific Purposes (CETS # 170) and followed guidelines provided in GOST 33216-2014 Rules for Working with Laboratory Rodents and Rabbits.
Received: 2021-06-30
Accepted: 2021-07-21
Published online: 2021-08-04
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Fig. 1. Foci of tuberculous lesions in the lung of a rabbit. A. Lung area under tuberculous granulomatous inflammation with distelectasis (micropreparation, stained with hematoxylin and eosin; ×40). B. Acid-resistant mycobacteria in the focus of tuberculous inflammation (micropreparation, staining according to Ziehl–Neelsen; ×1000). C. Lung: a focus of caseous necrosis with leukocyte infiltration and perifocal macrophage-epithelioid granulomas (micropreparation, stained with hematoxylin and eosin; ×100)
Fig. 2. There are multiple tuberculous tubercles on the parietal peritoneum of a rabbit-disseminates (circled in a round frame) (macro specimen)
Fig. 3. Tuberculous peritonitis in rabbits. Micropreparation. A. Tuberculous granulomatous peritonitis (granulomas are shown by arrows) (staining with hematoxylin and eosin; ×200). B. Caseous peritonitis (a focus of caseous necrosis is shown by the arrow) (stained with hematoxylin and eosin; ×100). C. Accumulations of ironcontaining pigment in necrotic masses and in adjacent macrophages (iron-containing pigment is blue-green, some of the macrophages with pigments are shown by arrows) (Perls reaction; ×100). D. Fragment of Fig. C (Perls reaction; ×200)
Fig. 4. Tuberculous peritonitis pathogenesis pattern
