ISSN Print 2500–1094
ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Retinal abnormalities in transgenic mice overexpressing aberrant human FUS[1-359] gene
About authors
1 Belgorod State National Research University, Belgorod, Russia
2 Institute of Physiologically Active Substances, Moscow, Russia
3 Kursk State Medical University, Kursk, Russia
4 Sechenov First Moscow State Medical University, Moscow, Russia
Correspondence should be addressed: Vladislav O. Soldatov
Pobedy, 85, 308015, Belgorod; moc.liamg@votadlosmrahp
About paper
Funding: the study was carried out with the financial support of the Russian Foundation for Basic Research within the framework of the scientific project № 19-315-90114.
Author contribution: Soldatov VO — the main idea, design of the experiment, ophthalmoscopy, writing an article, design of primers for gene expression assay; Kukharsky MS — the main idea, design of the experiment, writing an article, western blot analysis; Soldatova MO — RNA isolation, qPCR; Puchenkova OA — retina and spinal cord collection, RNA extraction, qPCR; Nikitina YuA — preparation of animal populations, genotyping, western blot analysis; Lysikova EA — preparation of animal populations, genotyping, western-blot analysis, writing an article; Kartashkina NL — interpretation and scoring of ophthalmoscopic picture; Deykin AV — consultation on the main idea and design of the study; Pokrovskiy MV — consultation on the main idea and design of the study.
Compliance with ethical standards: animal procedures were approved by the local ethics committee of the Belgorod State National Research University (protocol № 5 / 19–25 dated september 25. 2019). All manipulations were carried out in compliance with the requirements of the International Recommendations of European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes (1997).
Received: 2021-08-19
Accepted: 2021-08-28
Published online: 2021-08-31
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Fig. 1. Results of ophthalmoscopic examination. A. Representative ophthalmoscopic pictures of experimental animals. B. Results of scale scoring of ophthalmoscopic picture
Fig. 2. Absence of truncated FUS protein in retinal tissues of transgenic mice
Fig. 3. Normalized heatmap for relative expression of genes related to inflammation (Vegfa, Icam1, Il6, Il1b, and Tnfa), neuroplasticity (Bdnf), autophagy (Atg7, Atg5), and regulation of apoptosis (Bax, Bcl2)
Table. Primers, used for gene expression assay
