ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Predictive potential of macrophage population phenotyping in malignization of H. pylori-associated chronic gastritis
About authors
1 V.I. Vernadsky Crimean Federal University, Simferopol, Russia
2 Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia
Correspondence should be addressed: Tatiana P. Sataieva
Bulvar Lenina, 5/7, Simferopol, 295006; ur.liam@loocznat
About paper
Funding: the study was carried out within the framework of the Government Assignment № FZEG-2020-0060 of the Ministry of Science and Higher Education of the Russian Federation in the field of scientific research “Algorithms for molecular genetic diagnosis of malignant neoplasms and approaches to their targeted therapy using cellular and genetic technologies”.
Author contribution: Golubinskaya EP — clinical data analysis, immunohistochemistry, manuscript editing; Sataieva TP, Fomochkina II — systematic analysis, manuscript writing; Kubyshkin AV — statistical analysis, manuscript editing; Makalish TP — sample preparation for morphological assessment, immunohistochemistry; Shkolyar NA — biopsy sample collection and preparation; Galyshevskaya AA, Varghese DV — morphometric data processing.
Compliance with ethical standards: the study was approved by the Ethics Committee of the Medical Academy named after S. I. Georgievsky (protocol № 15 dated December 5, 2020); the study was conducted in accordance with the Declaration of Helsinki 1964 (revised in 1975 and 1983), Good Clinical Practice (GCP) standards and the Federal Law № 323-FZ “On the Basics of Protecting the Health of Citizens in the Russian Federation” dated November 21, 2011. The informed consent was submitted by all patients.
Received: 2021-08-19
Accepted: 2021-08-03
Published online: 2021-09-14
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Fig. 1. Gastric mucosal pattern in controls. А. Regularly shaped columnar epithelium forming the gastric pits, hematoxylin and eosin stain (×10). B. СD163-positive cytoplasmic stain in solitary type 2 monocytes/macrophages, IHC (×40). C. СD68-positive cytoplasmic stain in general macrophage population, IHC (×40). D. СD31-positive cytoplasmic stain in small vessel endothelium, IHC (×40)
Fig. 2. Non-atrophic chronic gastritis. А. Moderate lymphoplasmacytic infiltration with admixed solitary leukocytes in the lamina propria of the antral mucosa, increased mucoidization of the columnar epithelium of the integument and pits, hematoxylin and eosin stain (×10). B. СD163-positive cytoplasmic stain in type 2 monocytes/ macrophages, IHC (×40). C. СD68-positive cytoplasmic stain in general macrophage population, IHC (×10). D. СD31-positive cytoplasmic stain in blood vessel endothelium, IHC (×40)
Fig. 3. Moderate subactive metaplastic atrophic chronic gastritis. А. Large intestinal metaplasia, hematoxylin and eosin stain (×10). B. СD163-positive cytoplasmic stain in type 2 monocytes/macrophages within lamina propria mucosae, IHC (×40). C. СD68-positive cytoplasmic stain in macrophages within lamina propria mucosae, IHC (×40). D. СD31-positive cytoplasmic stain in blood vessel endothelium, IHC (×40)
Fig. 4. Adenocarcinomas. А. Tubular adenocarcinoma with ulceration, hematoxylin and eosin stain (×10). B. СD163-positive cytoplasmic stain, IHC (×100). C. СD68positive cytoplasmic stain, IHC (×100). D. СD31-positive cytoplasmic stain, IHC (×200)
Table. Immunohistochemical study of macrophages and microvascular bed in patients with chronic gastritis and adenocarcinoma
Note: * — significant differences with the control group, p ≤ 0.05.
