ORIGINAL RESEARCH

Microbiota of semen samples with normozoospermia: analysis of real-time PCR data

About authors

1 Ural State Medical University, Yekaterinburg, Russia

2 Medical Center “Garmonia”, Yekaterinburg, Russia

3 Yeltsin Ural Federal University, Yekaterinburg, Russia

4 Institute of Mathematics and Mechanics, Yekaterinburg, Russia

5 Ivanovo State Medical Academy, Ivanovo, Russia

Correspondence should be addressed: Еkaterina S. Voroshilina
Repina, 3, Yekaterinburg, 620014; moc.liamg@anilihsorov

About paper

Acknowledgments: the authors would like to thank VN Khayutin, director of “Garmonia” Medical Center, for allowing them to conduct the study in the clinic's laboratory department.

Author contribution: Voroshilina ES — organization of the study, data analysis, writing the article; Zornikov DL — data analysis, writing the article; Ivanov AV — statistical processing, data analysis, writing the article; Pochernikov DG — patient selection, writing the article; Panacheva EA — literature review, data analysis, patient selection, conducting semen analyses and PCR tests, writing the article.

Compliance with ethical standards: the study was approved by the Ethics Committee of Ural State Medical University, Federal State Budget Educational Institution of Higher Education under the Ministry of Health of the Russian Federation (Protocol № 7 dated September 20, 2019). All patients signed the informed written consent to participation in the study.

Received: 2021-09-16 Accepted: 2021-10-06 Published online: 2021-10-28
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  1. Baud D, Pattaroni C, Vulliemoz N, Castella V, Marsland BJ, Stojanov M. Sperm Microbiota and Its Impact on Semen Parameters. Front Microbiol. 2019;10:234. Published 2019 Feb 12. DOI: 10.3389/fmicb.2019.00234.
  2. EAU Guidelines. Edn. presented at the EAU Annual Congress Milan Italy 2021. Available from: https://uroweb.org/guideline/ urological-infections/.
  3. Ochsendorf FR. Sexually transmitted infections: impact on male fertility. Andrologia. 2008; 40 (2): 72–5. DOI: 10.1111/j.14390272.2007.00825.x.
  4. Schuppe HC, Pilatz A, Hossain H, Diemer T, Wagenlehner F, Weidner W. Urogenital Infection as a Risk Factor for Male Infertility. Dtsch Arztebl Int. 2017; 114 (19): 339–46. DOI: 10.3238/ arztebl.2017.0339.
  5. Baud D, Vulliemoz N, Ammerdorffer A, Gyger J, Greub G, Castella V, et al. Waddlia chondrophila, a Chlamydia-related bacterium, has a negative impact on human spermatozoa. Hum Reprod. 2018; 33 (1): 3–10. DOI: 10.1093/humrep/dex342.
  6. Hou D, Zhou X, Zhong X, Settles ML, Herring J, Wang L, et al. Microbiota of the seminal fluid from healthy and infertile men. Fertil Steril. 2013; 100 (5): 1261–9. DOI: 10.1016/j. fertnstert.2013.07.1991.
  7. Liu CM, Osborne BJ, Hungate BA, Liu CM, Osborne BJ, Hungate BA, et al. The semen microbiome and its relationship with local immunology and viral load in HIV infection. PLoS Pathog. 2014; 10 (7): e1004262. DOI: 10.1371/journal.ppat.1004262.
  8. Mändar R, Punab M, Korrovits P, Türk S, Ausmees K, Lapp E, et al. Seminal microbiome in men with and without prostatitis. Int J Urol. 2017; 24 (3): 211–6. DOI: 10.1111/iju.13286.
  9. Weng SL, Chiu CM, Lin FM, Huang WC, Liang C, Yang T, et al. Bacterial communities in semen from men of infertile couples: metagenomic sequencing reveals relationships of seminal microbiota to semen quality. PLoS One. 2014; 9 (10): e110152. DOI: 10.1371/journal.pone.0110152.
  10. Štšepetova J, Baranova J, Simm J, Parm Ü, Rööp T, Sokmann S, et al. The complex microbiome from native semen to embryo culture environment in human in vitro fertilization procedure. Reprod Biol Endocrinol. 2020; 18 (1): 3. DOI: 10.1186/s12958-019-0562-z.
  11. Kiessling AA, Desmarais BM, Yin HZ, Loverde J, Eyre RC. Detection and identification of bacterial DNA in semen. Fertil Steril. 2008; 90 (5): 1744–56. DOI: 10.1016/j.fertnstert.2007.08.083.
  12. Voroshilina ES, Zornikov DL, Panacheva EA. Evaluation of the ejaculate microbiota by real-time PCR and culture-based technique. Bulletin of RSMU. 2019; 1: 41–6. DOI: 10.24075/ brsmu.2019.009.
  13. Pochernikov DG, Vitvickaya YuG, Boldyreva MN, Galkina IS. Informativnost' biomateriala dlja issledovanija mikrobioty urogenital'nogo trakta muzhchin metodom PCR-RV (pilotnoe issledovanie). Jeksperimental'naja i klinicheskaja urologija. 2019; 2: 128–133. DOI: 10.29188/2222-8543-2019-11-2-128-132. Russian.
  14. WHO. Laboratory manual for the examination and processing of human semen. 5th ed. Geneva: WHO; 2010.
  15. Jain AK, Murty MN, Flynn PJ. Data clustering: a review. ACM computing surveys (CSUR). 1999; 31 (3): 264–323.
  16. Arthur D, Vassilvitskii S. K-means++: the advantages of careful seeding. In: Hal Gabow, editor. Proceedings of the Eighteenth Annual ACM-SIAM Symposium on Discrete Algorithms (Proceedings in Applied Mathematics) 18th ed. Society for Industrial and Applied Mathematics (3 January 2007); p. 1027– 35. DOI: 10.1145/1283383.1283494.
  17. Rousseeuw PJ. Silhouettes: A graphical aid to the interpretation and validation of cluster analysis. J Comput Appl Math. 1987; 20; 53–65.
  18. Davies DL, Bouldin DW. A Cluster Separation Measure. IEEE Transactions on Pattern Analysis and Machine Intelligence. PAMI1. 1979; 2: 224–7.
  19. de Goffau MC, Lager S, Salter SJ, Wagner J, Kronbichler A, Charnock-Jones DS, et al. Recognizing the reagent microbiome. Nat Microbiol. 2018; 3 (8): 851–3.
  20. Yang H, Zhang J, Xue Z, Zhao C, Lei L, Wen Y, et al. Potential Pathogenic Bacteria in Seminal Microbiota of Patients with Different Types of Dysspermatism. Sci Rep. 2020;10 (1): 6876. DOI: 10.1038/s41598-020-63787-x.
  21. Voroshilina ES, Zornikov DL, Ivanov AV, Pochernikov DG, Panacheva EA. Semen microbiota: cluster analysis of real-time PCR data. Bulletin of RSMU. 2020; 5: 62–70. DOI: 10.24075/ brsmu.2020.064.
  22. Ivanov IB, Kuzmin MD, Gritsenko VA. Microflora of the seminal fluid of healthy men and men suffering from chronic prostatitis syndrome. Int J Androl. 2009; 32 (5): 462–7. DOI: 10.1111/j.13652605.2008.00878.x.
  23. Pochernikov DG, Postovoytenko NT, Getman VV, Galkina IS. Diagnostic significance of Lactobacillus spp. identification in ejaculate. Bulletin of RSMU. 2020; 3: 38–45. DOI: 10.24075/ brsmu.2020.039.
  24. Lipsky BA, Byren I, Hoey CT. Treatment of bacterial prostatitis. Clin Infect Dis. 2010; 50 (12): 1641–52. DOI: 10.1086/652861.