2022 / 02

DOI: 10.24075/brsmu.2022.023

ORIGINAL RESEARCH

Development of a recombinant oncolytic poliovirus type 3 strain with altered cell tropism

Hamad A1,2,3, Soboleva AV1, Vorobyev PO1, Mahmoud M1,2, Vasilenko KV4, Chumakov PM1, Lipatova AV1
About authors

1 Engelhardt Institute of Molecular Biology, Moscow, Russia

2 Moscow Institute of Physics and Technology (MIPT), Moscow, Russia

3 Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Moscow, Russia

4 Pirogov Russian National Research Medical University, Moscow, Russia

Correspondence should be addressed: Anastasia V. Lipatova
Vavilova, 32/1, Moscow, 119991, Russia; moc.liamg@vnaavotapil

About paper

Funding: the project was supported by the Russian Science Foundation (agreement number 20-75-10157 of 14 August 2020 “Research on the possibilities of obtaining recombinant strains of oncolytic viruses with tumor-specific replication and immunomodulatory protein expression”).

Author contribution: Chumakov PM, Lipatova AV — study concept and planning, data analysis; Hamad A, Vorobyev PO, Soboleva AV, Mahmoud M, Vasilenko KV, and Lipatova AV — laboratory experimental work; Hamad A, Soboleva AV, and Lipatova AV — preparation of figures and data interpretation.

Compliance with ethical standards: the study was conducted per the requirements of the World Medical Association Declaration of Helsinki 2000 and its subsequent revisions; in compliance with the principles of the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes.

Received: 2022-04-18 Accepted: 2022-04-28 Published online: 2022-04-30
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Fig. 1. A scheme of 5′ UTR in RVP3. The chimeric region combines sequences of rhinovirus and poliovirus type 3
Fig. 2. Comparison of viral titers for enterovirus-sensitive cell cultures RD and HEK293T
Fig. 3. Results of anti-poliovirus type 3 neutralization microtests for different viral strains
Fig. 4. In vitro functional tests for RVP3 and PV3S. A. Cell line sensitivity to infection. B. Viral replication capacity
Table. Oligonucleotide sequences used for RVP3 DNA amplification