2022 / 02

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DOI: 10.24075/brsmu.2022.014

ORIGINAL RESEARCH

Regenerative effects of Gly-His-Lys and Gly-His-Lys-D-Ala peptides in infected skin wounds

Rakhmetova KK, Mishina ES, Vorvul AO, Bobyntsev II, Dolgintsev ME, Bezhin AI
About authors

Kursk State Medical University, Kursk, Russia

Correspondence should be addressed: Igor I. Bobyntsev
Karla Marxa, 3, Kursk, 305041, Russia; ur.liam@gibob

About paper

Author contribution: Rakhmetova KK — sample collection, concept and design of the study, data analysis and interpretation, writing of the manuscript; Mishina ES — histological and morphological examination, data analysis and interpretation; Vorvul AO — statistical processing of the data, writing of the manuscript; Bobyntsev II — concept and design of the study, scientific editing of the manuscript; Dolgintsev ME — data analysis and interpretation, writing of the manuscript; Bezhin AI — concept and design of the study.

Compliance with ethical standards: the study was approved by Ethics Committee of the Kursk State Medical University (Protocol № 1 of January 16, 2014). The study was carried out in compliance with the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes and the Guidelines for conducting preclinical drug trials (Moscow, 2012).

Received: 2022-02-10 Accepted: 2022-03-01 Published online: 2022-04-13
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  1. Bashkina OA, Samotrueva MA, Azhikova AK, Paknnova LR. Neuroimmunoendocrine regulation of the skin functioning. Meditsinskaya Immunologiya. 2019; 21 (5): 807–20. DOI: 10.15789/1563-0625-2019-5-807-820. Russian.
  2. Makarevich PI, Efimenko AY, Tkachuk VA Biochemical regulation of regenerative processes by growth factors and cytokines: basic mechanisms and relevance for regenerative medicine. Biochemistry (Moscow). 2020; 85 (1): 11–26. DOI: 10.1134/ S0006297920010022. PubMed PMID: 32079514.
  3. Khavinson VKh. Peptide medicines: past, present, future. Clinical Medicine (Russian Journal). 2020; 98 (3): 165–177. DOI: 10.30629/0023-2149-2020-98-3-165-177. Russian.
  4. Siméon A, Wegrowski Y, Bontemps Y, Maquart FX. Expression of glycosaminoglycans and small proteoglycans in wounds: modulation by the tripeptide-copper complex glycyl-L-histidylL-lysine-Cu(2+). J Invest Dermatol. 2000; 115 (6): 962–8. DOI: 10.1046/j.1523-1747.2000.00166.x. PubMed PMID: 11121126
  5. Pickart L, Margolina A. Regenerative and Protective Actions of the GHK-Cu Peptide in the Light of the New Gene Data. Int J Mol Sci. 2018; 19 (7): 1987. DOI: 10.3390/ijms19071987. PubMed PMID: 29986520.
  6. Pickart L, Vasquez-Soltero JM, Margolina A. GHK Peptide as a Natural Modulator of Multiple Cellular Pathways in Skin Regeneration. Biomed Res Int. 2015; 2015: 648108. DOI: 10.1155/2015/648108. PubMed PMID: 26236730.
  7. Ahmed MR, Basha SH, Gopinath D, Muthusamy R, Jayakumar R. Initial upregulation of growth factors and inflammatory mediators during nerve regeneration in the presence of cell adhesive peptide-incorporated collagen tubes. J Peripher Nerv Syst. 2005; 10 (1): 17–30. DOI: 10.1111/j.1085-9489.2005.10105.x. PubMed PMID: 15703015.
  8. Mazurowska L, Mojski M. Biological activities of selected peptides: skin penetration ability of copper complexes with peptides. J Cosmet Sci. 2008; 59 (1): 59–69. PubMed PMID: 18350235
  9. Pickart L, Vasquez-Soltero JM, Margolina A. The human tripeptide GHK-Cu in prevention of oxidative stress and degenerative conditions of aging: implications for cognitive health. Oxid Med Cell Longev. 2012; 2012: 324832. DOI: 10.1155/2012/324832. PubMed PMID: 22666519.
  10. Siméon A, Emonard H, Hornebeck W, Maquart FX. The tripeptidecopper complex glycyl-L-histidyl-L-lysine-Cu2+ stimulates matrix metalloproteinase-2 expression by fibroblast cultures. Life Sci. 2000; 67 (18): 2257–65. DOI: 10.1016/s0024-3205(00)00803-1. PubMed PMID: 11045606.
  11. Kurtseva AA, Smakhtin MYu, Ivanov AV, Besedin AV, Cherdakov VYu. The effects of Gly-His-Lys peptide on neutrophil functions and its regenerative activity in skin wounds. Kursk scientific and practical bulletin “Man and his health”. 2008; (1): 36–40. Russian.
  12. R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. 2020. Available from: https://www.R-project.org/.
  13. Pickart L, Vasquez-Soltero JM, Margolina A. GHK-Cu may Prevent Oxidative Stress in Skin by Regulating Copper and Modifying Expression of Numerous Antioxidant Genes. Cosmetics. 2015; 2 (3): 236–47. DOI: 10.3390/cosmetics2030236.
  14. McCormack MC, Nowak KC, Koch RJ. The effect of copper peptide and tretinoin on growth factor production in a serumfree fibroblast model. Arch Facial Plast Surg. 2001; 3 (1): 28–32. PubMed PMID: 11176716.
  15. Rakhmetova KK, Dolgintsev ME, Bobyntsev II, Bezhin AI, Vorvul AO, Belykh AE. Effect of GHK-D-Ala peptide on innate immunity mechanisms and lipid peroxidation processes in infected wounds. Humans and their Health. 2021; 24 (1): 54–61. DOI: 10.21626/vestnik/2021-1/07. Russian.
  16. Bobyntsev II, Chernysheva OI, Dolgintsev ME, Smakhtin MY, Belykh AE. Anxiolytic effects of Gly-His-Lys peptide and its analogs. Bull Exp Biol Med. 2015; 158 (6): 726–8. DOI: 10.1007/s10517-015-2847-3. PubMed PMID: 25900608.
  17. Bobyntsev II, Chernysheva OI, Dolgintsev ME, Smakhtin MYu, Belykh AE. Effect of Gly-His-Lys peptide and its analogs on pain sensitivity in mice. Eksper. i klin. farmakol. 2015; 78 (1): 13–15. DOI: 10.30906/0869-2092-2015-78-1-13-15. Russian.
  18. Wang X, Liu B, Xu Q, Sun H, Shi M, Wang D et al. GHK-Culiposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis. Wound Repair Regen. 2017; 25 (2): 270–8. DOI: 10.1111/wrr.12520. PubMed PMID: 28370978.
  19. Gruchlik A, Chodurek E, Dzierzewicz Z. Effect of Gly-His-Lys and its copper complex on TGF-β secretion in normal human dermal fibroblasts. Acta Pol Pharm. 2014; 71 (6): 954–8. PubMed PMID: 25745767.
  20. Gruchlik A, Jurzak M, Chodurek E, Dzierzewicz Z. Effect of GlyGly-His, Gly-His-Lys and their copper complexes on TNF-alphadependent IL-6 secretion in normal human dermal fibroblasts. Acta Pol Pharm. 2012; 69 (6): 1303–6. PubMed PMID: 23285694.