2022 / 04

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DOI: 10.24075/brsmu.2022.040

ORIGINAL RESEARCH

EPOR/CD131-mediated attenuation of rotenone-induced retinal degeneration is associated with upregulation of autophagy genes

Soldatov VO1, Pokrovskiy MV1, Puchenkova OA1, Zhunusov NS1, Krayushkina AM1, Grechina AV2, Soldatova MO3, Lapin KN4, Bushueva OYu3
About authors

1 Belgorod State National Research University, Belgorod, Russia

2 Sechenov First Moscow State Medical University, Moscow, Russia

3 Kursk State Medical University, Kursk, Russia

4 Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia

Correspondence should be addressed: Vladislav O. Soldatov
Pobedy, 85, Belgorod, 308015, Russia; moc.liamg@votadlosmrahp

About paper

Funding: State Assignment "Laboratory for Genome Editing in Biomedicine and Veterinary FZWG-2021-0016".

Author contribution: Soldatov VO — concept and design of the study, writing of the manuscript; Pokrovsky MV, Lapin KN — consulting on the concept and design of the study; Puchenkova OA — collection of samples for histological examination and target gene expression analysis; Puchenkova OA, Zhunusov NS — modeling of the rotenone-induced retinal degeneration; Zhunusov NS, Krayushkina AM — in vitro assay of rotenone cytoprotective activity; Grechina AV — morphological study; Bushueva OYu, Soldatova MO — RNA extraction, quantitative PCR assay.

Compliance with ethical standards: the study was approved by the ethical committee of the Belgorod State National Research University (Protocol № 06-07/21 of 15 July 2021); all procedures were performed in accordance with the Regulations on Laboratory Practice in the Russian Federation of 2003, in compliance with the 86/609 EEC Directive and ARRIVE guidelines.

Received: 2022-07-11 Accepted: 2022-08-01 Published online: 2022-08-12
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Fig. 1. A scheme of in vivo experiments to assess retinoprotective effects of EPOR/CD131 agonists in rotenone-induced retinopathy
Fig. 2. The therapeutic effect of pHBSP and EPO in the rotenone-induced retinopathy in rats. A. Representative histological images of the retina. B. The inner plexiform layer thickness measurements. C. Nuclei counts in the ganglion cell layer. D. Normalized heat map of gene expression levels for inflammation (Il6, Il1b), autophagy (Map1Lc3a, Atg7), mitophagy (Becn1, Prkn, Bnip3) and retinal degeneration (Nefl) markers. GCL — ganglion cell layer; IPL — inner plexiform layer; * — p < 0.05; * * — p < 0.005; * * * — p < 0.0005 (Kruskal–Wallis test, posthoc Dunn's test).
Fig. 3. Cytoprotective effects of pHBSP and EPO on the primary neuroglial cultures treated with 2.5 µМ rotenone. * * — p < 0.005; * * * — p < 0.0005 (Dunn's test)
Table. Primers used in qPCR target gene expression assay