ORIGINAL RESEARCH

Androgen levels in blood and follicular fluid of IVF patients with diminished ovarian reserve

About authors

Kulakov national medical research center for obstetrics, gynecology and perinatology, Moscow, Russia

Correspondence should be addressed: Alla A. Gavisova
Akademika Oparina, 4, Moscow, 117997; ur.ay@allaivag

About paper

Author contribution: Gavisova AA, Dolgushina NV — stady concept and design; Gavisova AA, Shevtsova MA — analysis, manuscript writing; Kindysheva SV — laboratory tests, statistial analysis; Starodubtseva NL, Nazarenko TA, Frankevich VE, Dolgushina NV — manuscript editing.

Compliance with ethical standards: the study was approved by the ethical review board at the Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology (protocol № 140 of 15 December 2014). The informed consent was submitted by all study participants.

Received: 2022-07-29 Accepted: 2022-08-13 Published online: 2022-08-19
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  1. Hill MJ, Levens ED, Levy G, Ryan ME, Csokmay JM, DeCherne AH, et al. The use of recombinant luteinizing hormone in patients undergoing assisted reproductive techniques with advanced reproductive age: a systematic review and meta-analysis. Fertil Steril. 2012; 97 (5): 1108–14.
  2. Luo S, Li S, Li X, Qin L, Jin S. Effect of pretreatment with transdermal testosterone on poor ovarian responders undergoing IVF/ICSI: a meta-analysis. Exp Ther Med. 2014; 8 (1): 187–94.
  3. Von Wolff M, Kollmann Z, Flück CE, Stute P, Marti U, Weiss B, et al. Gonadotrophin stimulation for in vitro fertilization significantly alters the hormone milieu in follicular fluid: a comparative study between natural cycle IVF and conventional IVF. Hum Reprod. 2014; 29 (5): 1049–57.
  4. Fanchin R, Mendez Lozano DH, Frydman N, Gougeon A, di Clemente N, Frydman R, et al. Anti-Müllerian hormone concentrations in the follicular fluid of the preovulatory follicle are predictive of the implantation potential of the ensuing embryo obtained by in vitro fertilization. J Clin Endocrinol Metab. 2007. 92 (5): 1796–802.
  5. Pabuccu R, Kaya C, Cağlar GS, Oztas E, Satiroglu H. Follicularfluid anti-Mullerian hormone concentrations are predictive of assisted reproduction outcome in PCOS patients. Reprod Biomed Online. 2009; 19 (5): 631–37.
  6. Takahashi C, Fujito A, Kazuka M, Sugiyama R, Ito H, Isaka K. Anti-Müllerian hormone substance from follicular fluid is positively associated with success in oocyte fertilization during in vitro fertilization. Fertil Steril. 2008; 89 (3): 586–91.
  7. Van Disseldorp J, Faddy MJ, Themmen AP, de Jong FH, Peeters PH, van der Schouw YT. Relationship of serum antimüllerian hormone concentration to age at menopause. J Clin Endocrinol Metab. 2008; 93 (6): 2129–34.
  8. Barbieri RL, Sluss PM, Powers RD, McShane PM, Vitonis A, Ginsburg E, et al. Association of body mass index, age, and cigarette smoking with serum testosterone levels in cycling women undergoing in vitro fertilization. Fertil Steril. 2005; 83 (2): 302–8.
  9. Kushnir M, Naessen T, Wanggren K, Hreinsson J, Rockwood AL, Meikle AW. Exploratory study of the association of steroid profiles in stimulated ovarian follicular fluid with outcomes of IVF treatment. J Steroid Biochem Mol Biol. 2016; 162: 126–33.
  10. Kim J, Lee J, Chang H, Jee BC, Chang SS, Kim SH. Anti-Mullerian hormone levels in the follicular fluid of the preovulatory follicle: a predictor for oocyte fertilization and quality of embryo. J Korean Med Sci. 2014; 29 (9): 1266–70.
  11. Wen X, Li D, Tozer A, Suzanna MD, Ray KI. Estradiol, progesterone, testosterone profiles in human follicular fluid and cultured granulosa cells from luteinized pre-ovulatory follicles. Reprod Biol Endocrinol. 2010; 8: 117.
  12. Handelsman D. Mass spectrometry, immunoassay and valid steroid measurements in reproductive medicine and science. Hum Reprod. 2017; 32 (6): 1–4.
  13. Yang Z, Zhou W, Zhou C, Zhou Y, Liu X, Ding G, et al. Steroid metabolome profiling of follicular fluid in normo- and hyperandrogenic women with polycystic ovary syndrome. J Steroid Biochem Mol Biol Epub. 2021; 206: 105806.
  14. Bongrani A, Plotton I, Mellouk N, Ramé C, Guerif F, Froment P, et al. High androgen concentrations in follicular fluid of polycystic ovary syndrome women. Reprod Biol Endocrinol. 2022; 20 (1): 88.
  15. Harwood DT, Handelsman DJ. Development and validation of a sensitive liquid chromatography-tandem mass spectrometry assay to simultaneously measure androgens and estrogens in serum without derivatization. Clin Chim Acta. 2009; 409 (1–2): 78–84.
  16. Jonas Schäler, Georg Thaller, Dirk Hinrichs. A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria, 2018. R Studio: Integrated Development for R. R Studio, Inc., Boston MA.
  17. Poulsen LC, Englund ALM, Andersen AS, Bøtkjær JA, Mamsen LS, Damdimopoulou P, et al. Follicular hormone dynamics during the midcycle surge of gonadotropins in women undergoing fertility treatment. Mol Hum Reprod. 2020; 26 (4): 256–68.
  18. Burduli AG, Kitsilovskaya NA, Sukhova YV, Vedikhina IA, Ivanets TY, Chagovets VV, Starodubtseva NL, Frankevich VE. Follicular fluid and assisted reproductive technology programs outcomes (literature review). Gynecology. 2019; 21 (6): 36–40. Russian.
  19. Fuentes A, Sequeira K, Tapia-Pizarro A, Muñoz A, Salinas A, Céspedes P. et al. Androgens profile in blood serum and follicular fluid of women with poor ovarian response during controlled ovarian stimulation reveals differences amongst POSEIDON stratification groups: a pilot study. Front Endocrinol (Lausanne). 2019; 10: 458.