2022 / 04

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DOI: 10.24075/brsmu.2022.042

ORIGINAL RESEARCH

Prognostic significance of oral fluid fluoride measurement in acute pericoronitis

Vagner VD1, Sarf EA2, Belskaya LV2, Korshunov AS3, Kuryatnikov KN3, Bondar AA3, Meloyan AD3, Maksimenko KA3, Kasiy MN3
About authors

1 Central Research Institute of Dentistry and Maxillofacial Surgery, Moscow, Russia

2 Omsk State Pedagogical University, Omsk, Russia

3 Omsk State Medical University, Omsk, Russia

Correspondence should be addressed: Andrey S. Korshunov
Kosareva, 34, Omsk, 644043, Russia; ur.liam@885081_k_yerdna

About paper

Author contribution: Vagner VD — planning of the study, literature analysis, data interpretation; Sarf EA — biochemical research, statistical analysis; Belskaya LV — planning of the study, biochemical research, manuscript preparation; Korshunov AS — planning of the study, literature analysis, data interpretation, collection of clinical samples, manuscript preparation; Kuryatnikov KN — collection of clinical samples, data interpretation, manuscript preparation; Bondar AA, Meloyan AD, Maksimenko KA, Kasiy MN — sample preparation, data analysis.

Compliance with ethical standards: the study was approved by ethical review board of the Omsk State Medical University (Protocol № 113 of 26 November 2019); all participants or their representatives provided informed consent for participation in the study.

Received: 2022-08-12 Accepted: 2022-08-26 Published online: 2022-08-29
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Fig. 1. Calibration curve for fluoride measurement by capillary electrophoresis, built in a standard range of 0.1–10 mg/L
Fig. 2. А. Electropherogram for calibration solution containing a mixture of anions (1 — chlorides, 2 — nitrites, 3 — sulfates, 4 — nitrates, 5 — fluorides, 6 — phosphates). The insert shows fluoride ion peaks within the range of 0.25–10.0 mg/L. B. Electropherogram of saliva (100 µL), pure or mixed with 100 µL and 200 µL aliquots of fluoride standard solution (diluted 20-fold)
Fig. 3. Calibration curve for fluoride measurement by photometry
Fig. 4. Fluoride content for groups 1 and 2 before and after surgery (post-operative days 1 and 3) compared with the control group
Table 1. Electropherogram parameters for fluoride measurement
Table 2. Fluoride measurement for a selection of samples. CE, capillary electrophoresis