2022 / 06

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DOI: 10.24075/brsmu.2022.059

ORIGINAL RESEARCH

The role of mef and ermB drug resistance genetic markers in the selection of fecal microbiota donors

Gospodaryk AV1, Ulakhanova LA1, Esiev SS1, Polyakova EV1, Shansky YD1, Bespyatykh JA1,2,3
About authors

1 Lopukhin Federal Research and Clinical Center Of Physical-Chemical Medicine under the Federal Medical Biological Agency, Moscow, Russia

2 Mendeleev University of Chemical Technology of Russia, Moscow, Russia

3 Semashko National Research Institute of Public Health, Moscow, Russia

Correspondence should be addressed: Alina V. Gospodaryk
Krasnogorskoe shosse, 15, Odintsovo, Moscow region, 143007, Russia; gro.mcpcr@kiradopsog.anila

About paper

Funding: Grant of ANO Moscow Center for Innovative Technologies in Healthcare (#2412-31).

Author contribution: Gospodaryk AV — selection of study participants, PCR analysis of biological samples, literature analysis, article authoring; Ulakhanova LA — literature analysis, article authoring, fecal culture, bacteriological analysis; Esiev SS — literature analysis, isolation of nucleic acids from biological samples; Polyakova EV — practical part of the bacteriological analysis, isolation of nucleic acids from biological samples; Shansky YD — statistical data processing; Bespyatykh JA — study concept, consulting, article authoring and editing.

Compliance with ethical standards: the study was approved by the Ethics Committee of the Federal Research and Clinical Center Of Physical-Chemical Medicine under the Federal Medical Biological Agency of Russia (Minutes #2022/05/31 of May 31, 2022). All study participants signed the voluntary informed consent form.

Received: 2022-10-20 Accepted: 2022-11-25 Published online: 2022-12-16
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Table 1. Stool culture method
Table 2. Bacteria culture test, stool samples from potentially healthy volunteers
Table 3. Prevalence of mef and ermB resistance genes in feces, different age groups
Note: * — ratio of the number of samples in which the gene was detected to the total number of samples in the respective group.
Table 4. Prevalence of mefA and ermB resistance genes in the "mother-child" group
Note: * — ratio of the number of samples in which the gene was detected to the total number of samples in the respective group.