Funding: experimental studies of laser assisted hatching were performed using the unique scientific facility “Terawatt Femtosecond Laser Complex” in the Center for Collective Usage “Femtosecond Laser Complex” of JIHT RAS and were funded by the Ministry of Science and Higher Education of the Russian Federation (State Assignment № 075-01129-23-00). Embryos were obtained using the unique scientific facility Transgenebank and supported by the Ministry of Science and Higher Education of the Russian Federation (grant № 075-15-2021-668 of 29.07.2021).

Author contribution: Sitnikov DS — laser microsurgery, data processing, manuscript writing; Ilina IV — study concept, manuscript writing; Filatov MA — handling embryos, statistical processing, manuscript writing; Silaeva YuYu — management; discussion and manuscript editing — all authors.

Compliance with ethical standards: animals were handled in accordance with the Declaration of Helsinki and the guidelines issued by the Bioethics Commission at the Institute of Gene Biology of the Russian Academy of Sciences.