ISSN Print 2500–1094
ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Assessment of the zona pellucida microdissection on its thickness in mammalian embryos
About authors
1 Joint Institute for High Temperatures, Moscow, Russia
2 Institute of Gene Biology, Moscow, Russia
Correspondence should be addressed: Dmitry S. Sitnikov
Krasnokazarmennaya, 17а, Moscow, 111116, Russia; moc.liamg@sd.kintis
About paper
Funding: experimental studies of laser assisted hatching were performed using the unique scientific facility “Terawatt Femtosecond Laser Complex” in the Center for Collective Usage “Femtosecond Laser Complex” of JIHT RAS and were funded by the Ministry of Science and Higher Education of the Russian Federation (State Assignment № 075-01129-23-00). Embryos were obtained using the unique scientific facility Transgenebank and supported by the Ministry of Science and Higher Education of the Russian Federation (grant № 075-15-2021-668 of 29.07.2021).
Author contribution: Sitnikov DS — laser microsurgery, data processing, manuscript writing; Ilina IV — study concept, manuscript writing; Filatov MA — handling embryos, statistical processing, manuscript writing; Silaeva YuYu — management; discussion and manuscript editing — all authors.
Compliance with ethical standards: animals were handled in accordance with the Declaration of Helsinki and the guidelines issued by the Bioethics Commission at the Institute of Gene Biology of the Russian Academy of Sciences.
Received: 2022-11-09
Accepted: 2022-12-30
Published online: 2023-01-27
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Fig. 1. Scheme of femtosecond laser scalpel. 1 — femtosecond laser; 2 — converter to second harmonic; 3 — attenuator; 4 — telescope; 5 — mechanical laser interrupter; 6, 7 — mirrors for the laser beam wavelength; 8 — objective lens; 9 — motorized microscope stage; 10 — Petri dish containing embryo; 11 — substage condenser; 12 — substage lamp; 13 — CMOS camera; 14 — inverted microscope
Fig. 2. Embryos before zona microsurgery (А); zona pellucida after hatching (B). 1 — hole in the ZP after blastocyst hatching resulting from incision along the trajectory 1 (on the fragment а); 2 — results of incision along the trajectory 2 (on the fragment а). The zone after hatching of the embryo in the experimental group (C) and CC group (D)
Fig. 3. Changes in the ZP thickness during embryogenesis in the control (А) and experimental (B) groups
Fig. 4. Distribution of the ZP thinning coefficient in the experimental (А) and control (B) groups
