ORIGINAL RESEARCH
Survival of human cells in tissue-engineered constructs stored at room temperature
1 Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia
2 Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of the Federal Medical Biological Agency, Moscow, Russia
Correspondence should be addressed: Olga S. Rogovaya
Vavilova, 26, Moscow, 119334, Russia; ur.xednay@f62ayavogor
Funding: the study was supported by the Ministry of Science and Higher Education of the Russian Federation, Agreement № 075-15-2021-1063 of 28.09.2021.
Author contribution: Rogovaya OS, Eremeev AV — experimental procedure, data analysis; Alpeeva EV — data interpretation, literature review; Ruchko ES — experimental procedure; Vorotelyak EA — study planning.
Compliance with ethical standards: the study was approved by the Ethics Committee of the Koltzov Institute of Developmental Biology, RAS (protocol № 51 of 09 September 2021) and conducted in accordance with the principles of the WMA Declaration of Helsinki and its subsequent revisions.
- Utheim TP, Raeder S, Utheim OA, de la Paz M, Roald B, et al. Sterility control and long-term eye-bank storage of cultured human limbal epithelial cells for transplantation. Br J Ophthalmol. 2003; 93: 980–3. DOI: 10.1136/bjo.2008.149591.
- Mutsenko VV, Rogulska OY, Petrenko YA, Ehrlich H, Mazur SP, et al. Cryosensitivity of Mesenchymal Stromal Cells Cryopreserved Within Marine Sponge Ianthella basta Skeleton-Based Carriers. Probl Cryobiol Cryomedicine. 2016; 26: 13–23. DOI: 10.15407/cryo26.01.013.
- Petrenko Y, Petrenko A, Martin I, Wendt D. Perfusion bioreactorbased cryopreservation of 3D human mesenchymal stromal cell tissue grafts. Cryobiology. 2017; 76: 150–3. DOI: 10.1016/j.cryobiol.2017.04.001.
- Arutyunyan I, Elchaninov A, Sukhikh G, Fatkhudinov T. Cryopreservation of Tissue-Engineered Scaffold-Based Constructs: From Concept to Reality. Stem Cell Rev Rep. 2022; 18: 1234–52. DOI: 10.1007/s12015-021-10299-4.
- Knapik A, Kornmann K, Kerl K, Calcagni M, Contaldo C, et al. Practice of split-thickness skin graft storage and histological assessment of tissue quality. J Plast Reconstr Aesthet Surg. 2013; 66: 827–34. DOI: 10.1016/j.bjps.2013.02.003.
- Ge L, Sun L, Chen J, Mao X, Kong Y, et al. The viability change of pigskin in vitro. Burns. 2010; 36: 533–8. DOI: 10.1016/j.burns.2009.08.001.
- Rauen U, Kerkweg U, de Groot H. Iron-dependent vs. ironindependent cold-induced injury to cultured rat hepatocytes: a comparative study in physiological media and organ preservation solutions. Cryobiology. 2007; 54 (1): 77–86. DOI: 10.1016/j.cryobiol.2006.11.008.
- Rogovaya OS, Petrakova OS, Gvazava IG, Borisov MA, Vasilev AV. Issledovanie zhiznesposobnosti kul'tiviruemyx kletok cheloveka v suspenzii. Vestnik Moskovskogo universiteta. Seriya 16: biologiya. 2016. 3: 44–48.
- Fujita Ya, Nishimura M, Komori NW, Wada T, Shirakawa Ch, et al. A pair of cell preservation solutions for therapy with human adipose tissue-derived mesenchymal stromal cells. Regen Ther. 2020; 14: 95–102. DOI: 10.1016/j.reth.2019.10.004.
- Vackova I, Vavrinova E, Musilkova J, Havlas V, Petrenko Y. Hypothermic Storage of 3D Cultured Multipotent Mesenchymal Stromal Cells for Regenerative Medicine Applications. Polymers (Basel). 2022; 14 (13): 2553. DOI: 10.3390/polym14132553.
- Schulze K, López DA, Tillich UM, Frohme M. A simple viability analysis for unicellular cyanobacteria using a new autofluorescence assay, automated microscopy, and Image J. BMC Biotechnol. 2011; 11: 118. DOI: 10.1186/1472-6750-11-118.
- Tam E, McGrath M, Sladkova M, AlManaie A, Alostaad A, de Peppo GM. Hypothermic and cryogenic preservation of tissueengineered human bone. Ann NY Acad Sci. 2020; 1460 (1): 77– 87. DOI: 10.1111/nyas.14264.
- Sampaio-Pinto V, Janssen J, Chirico N, Serra M, Alves PM, et al. Roadmap to Cardiac Tissue-Engineered Construct Preservation: Insights from Cells, Tissues, and Organs. Adv Mater. 2021; 33 (27): e2008517. DOI: 10.1002/adma.202008517.
- Linkova DD, Rubtsova YP, Egorikhina MN. Cryostorage of Mesenchymal Stem Cells and Biomedical Cell-Based Products. Cells. 2022; 11 (17): 2691. DOI: 10.3390/cells11172691.
- Criswell T, Swart C, Stoudemire J, Brockbank K, Floren M, Eaker S, Hunsberger J. Shipping and Logistics Considerations for Regenerative Medicine Therapies. Stem Cells Transl Med. 2022; 11 (2): 107–13. DOI: 10.1093/stcltm/szab025.
- Jing D, Li C, Yao K, Xie X, Wang P, et al. The vital role of Gli1+ mesenchymal stem cells in tissue development and homeostasis. J Cell Physiol. 2021; 236 (9): 6077–89. DOI: 10.1002/jcp.30310.
- Krawczenko A, Klimczak A. Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Contribution to Angiogenic Processes in Tissue Regeneration. Int J Mol Sci. 2022; 23 (5): 2425. DOI: 10.3390/ijms23052425.