Rhegmatogenous retinal detachment complicated by macular hole is a rare disorder that is the most challenging in terms of vitreoretinal surgery, and good anatomical outcome is not always associated with high visual functions. Today, vitrectomy, involving macular hole closure with autologous platеlet-rich plasma, sealing peripheral retinal tears, and subsequent vitreal cavity tamponade with vitreous substitutes, is considered to be the most effective method for surgical treatment of this disorder. Despite variability of surgical approaches to treatment of rhegmatogenous retinal detachment complicated by macular holes, the search for safe and effective surgical technique, allowing one to achieve benificial morphological and functional outcome with minimal damage to the retinal structures and to minimize the patient's rehabilitation period, is still relevant. The clinical case reported demonstrates the possibility of performing single-stage endovitreal treatment of retinal detachment complicated by macular hole using the autologous conditioned plasma in combination with the short-term perfluorocarbon tamponade. The results of using this technique show its reliability and superior efficiency and ensure good morphological and functional outcome in the postoperative period: restored macular architectonics, macular hole closure, anatomic retinal adhesion, and improved visual functions.