ORIGINAL RESEARCH
ATOH1 factor expression induces rapid differentiation of iPSCs into neurons
1 Skolkovo Institute of Science and Technology, Center for Molecular and Cellular Biology, Moscow, Russia
2 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia
3 Pirogov Russian National Research Medical University, Moscow, Russia
4 Lomonosov Moscow State University, Moscow, Russia
Correspondence should be addressed: Nadya G. Gurskaya
Ostrovitianova, 1, str. 1, Moscow, 117997, Russia; ur.liam@ayaksrugn
Funding: the study was supported through the grant by RSF (№ 22-14-00141).
Author contribution: Stepanov AI — experimental procedure; Putlyaeva LV — experiment planning, manuscript writing; Didych DA — design and molecular cloning of the leniviral plasmid components, processing of figures; Galiakberova AA — cell culture; Gurskaya NG — concept and design of iPSC study; Lukyanov KA — general management.
- Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007; 131: 861–72.
- Chang C-Y, Ting H-C, Su H-L, Jeng J-R. Combining Induced Pluripotent Stem Cells and Genome Editing Technologies for Clinical Applications. Cell Transplant. 2018; 27: 379–92.
- Kleiman RJ, Engle SJ. Human inducible pluripotent stem cells: Realization of initial promise in drug discovery. Cell Stem Cell. 2021; 28: 1507–15.
- Madrid M, Sumen C, Aivio S, Saklayen N. Autologous Induced Pluripotent Stem Cell-Based Cell Therapies: Promise, Progress, and Challenges. Curr Protoc. 2021; 1: e88.
- Zhang N, Bailus BJ, Ring KL, Ellerby LM. iPSC-based drug screening for Huntington’s disease. Brain Res. 2016; 1638: 42–56.
- Chang C-Y, Ting H-C, Liu C-A, Su H-L, Chiou T-W, Lin S-Z, et al. Induced Pluripotent Stem Cell (iPSC)-Based Neurodegenerative Disease Models for Phenotype Recapitulation and Drug Screening. Molecules. 2020; 25. DOI: 10.3390/molecules25082000.
- Telias M. Neural differentiation protocols: how to choose the correct approach. Neural Regeneration Res. 2023; 18: 1273–4.
- Chambers SM, Fasano CA, Papapetrou EP, Tomishima M, Sadelain M, Studer L. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol. 2009; 27: 275–80.
- Zhang Y, Pak C, Han Y, Ahlenius H, Zhang Z, Chanda S, et al. Rapid single-step induction of functional neurons from human pluripotent stem cells. Neuron. 2013; 78: 785–98.
- Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy A, et al. A comprehensive library of human transcription factors for cell fate engineering. Nat Biotechnol. 2021; 39: 510–9.