Features of CD163+ and HLA-DR+ expression on blood monocytes associated with breast cancer

Patysheva MR1,2, Stakheyeva MN1,2, Grigoryeva ES2, Tarabanovskaya NA1, Bragina OD1, Kzhyshkowska JG2,3, Cherdyntseva NV1,2
About authors

1 Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia

2 National Research Tomsk State University, Tomsk, Russia

3 University of Heidelberg, Mannheim, Germany

Correspondence should be addressed: Marina R. Patysheva
per. Kooperativnyi, 7/1, Tomsk, 634009, Russia; ur.cmint.ocno@rm_avehsytap

About paper

Funding: the study was supported by the RSF grant (№ 22-25-00435).

Acknowledgments: we would like to express our gratitude to О.Е. Savelieva, the lead researcher at the Institute of Personalized Psychiatry and Neurology, Bekhterev National Research Medical Center for Psychiatry and Neurology (Saint Petersburg), for assistance in conducting flow cytometry.

Author contribution: Patysheva MR — study design, flow cytometry, data analysis, manuscript writing; Stakheyeva MN — study concept, study design, data analysis, manuscript writing; Grigoryeva ES — flow cytometry; Tarabanovskaya NA — selection of patients, patient clinical data acquisition; Bragina OD — selection of patients, patient clinical data acquisition; Kzhyshkowska JG — study concept, search for funding, discussion; Cherdyntseva NV — study design, discussion.

Compliance with ethical standards: the study was approved by the Ethics Committee of the Cancer Research Institute, Tomsk National Research Medical Center (protocol № 10 dated 05 December 2019). All subjects submitted the informed consent to study participation.

Received: 2023-09-14 Accepted: 2023-10-10 Published online: 2023-10-31

Circulating monocytes are important actors of the tumor growth pathogenesis. It has been shown that some features of the monocyte populations expressing endocytosis receptors or the major histocompatibility complex components are observed in blood of patients with breast cancer. The study was aimed to assess the relationship of tumor parameters and blood cytokine profile with the composition of circulating monocyte populations in patients with localized and locally advanced breast cancer. The study has shown that the circulating monocytes’ phenotypic characteristics are correlated to the clinical and morphological features of the neoplastic process. The content of populations with the CD14+CD16++CD163+ and CD14++CD16+CD163+ phenotypes positively correlates with the disease stage, while larger primary tumor size is associated with lower CD14+CD16++ monocyte levels. Elevated serum levels of IL8 and МСР-1 are observed in breast cancer patients. High IL6 levels in patients with breast cancer are associated with the reduced percentage of CD14++CD16-HLA-DR+, CD14+CD16++HLA-DR+ and CD14++CD16-СD163+ monocytes. Thus, CD163+ and HLA-DR+ monocytes are associated with the clinical and morphological parameters and blood levels of cytokines, which suggests that these populations are involved in breast cancer progression and indicates that further research is advisable for translation of the findings into clinical practice.

Keywords: breast cancer, monocytes, IL6, IL8, CD163, HLA-DR, MCP-1