Features of CD163+ and HLA-DR+ expression on blood monocytes associated with breast cancer

Patysheva MR1,2, Stakheyeva MN1,2, Grigoryeva ES2, Tarabanovskaya NA1, Bragina OD1, Kzhyshkowska JG2,3, Cherdyntseva NV1,2
About authors

1 Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia

2 National Research Tomsk State University, Tomsk, Russia

3 University of Heidelberg, Mannheim, Germany

Correspondence should be addressed: Marina R. Patysheva
per. Kooperativnyi, 7/1, Tomsk, 634009, Russia; ur.cmint.ocno@rm_avehsytap

About paper

Funding: the study was supported by the RSF grant (№ 22-25-00435).

Acknowledgments: we would like to express our gratitude to О.Е. Savelieva, the lead researcher at the Institute of Personalized Psychiatry and Neurology, Bekhterev National Research Medical Center for Psychiatry and Neurology (Saint Petersburg), for assistance in conducting flow cytometry.

Author contribution: Patysheva MR — study design, flow cytometry, data analysis, manuscript writing; Stakheyeva MN — study concept, study design, data analysis, manuscript writing; Grigoryeva ES — flow cytometry; Tarabanovskaya NA — selection of patients, patient clinical data acquisition; Bragina OD — selection of patients, patient clinical data acquisition; Kzhyshkowska JG — study concept, search for funding, discussion; Cherdyntseva NV — study design, discussion.

Compliance with ethical standards: the study was approved by the Ethics Committee of the Cancer Research Institute, Tomsk National Research Medical Center (protocol № 10 dated 05 December 2019). All subjects submitted the informed consent to study participation.

Received: 2023-09-14 Accepted: 2023-10-10 Published online: 2023-10-31
Fig. 1. Cytometry gating strategy for identification of monocyte subpopulations
Fig. 2. Association of blood cytokine levels with circulating monocyte phenotype in breast cancer. А. Serum cytokine levels in patients with breast cancer and healthy women. B. Correlation of serum IL6 levels with the CD163+ and HLA-DR+ monocyte counts in breast cancer
Table 1. Clinical and morphological parameters of patients with breast cancer
Table 2. Correlation of individual features and tumor clinical and morphological parameters with the monocyte subpopulations in patients with BC
Note: BMI — body mass index.
Table 3. Association of the disease stage with phenotypic characteristics of monocytes in patients with BC
Table 4. Phenotypic characteristics of blood monocytes depending on the tumor molecular subtype
Note: TNBC — triple-negative breast cancer, Lum В — luminal В breast cancer, Her2+ –Her2neu-positive breast cancer