2024 / 01

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DOI: 10.24075/brsmu.2024.005

ORIGINAL RESEARCH

Effect of probenecid on astrocyte activation in vitro

Babkina II1, Mazeeva VV2, Morozova MP1, Gorbacheva LR1,2
About authors

1 Pirogov Russian National Research Medical University, Moscow, Russia

2 Lomonosov Moscow State University, Moscow, Russia

Correspondence should be addressed: Lyubov R. Gorbacheva
Ostrovitianova, 1, Moscow, 117997, Russia; ur.liam@76ibrog

About paper

Funding: The study was supported by the Russian Science Foundation, Project № 22-25-00848.

Author contribution: Babkina II, Morozova MP — obtaining and maintaining primary culture of astrocytes; collection, interpretation and statistical analysis of data, manuscript writing; Mazeeva VV — obtaining and maintaining primary culture of astrocytes, interpretation and statistical analysis of data; Gorbacheva LR — conception and design of the experiment, interpretation of data, project management, manuscript writing.

Compliance with ethical standards: the study was approved by the Ethical Committee of Pirogov Russian National Research Medical University (Protocol № 23/2021 dated December 13, 2021).

Received: 2023-12-13 Accepted: 2024-01-24 Published online: 2024-02-26
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Fig. 1. Effect of Panx1 blockade on survival of thrombin- or LPS-activated astrocytes. * — р < 0.05, # — р < 0.05 (compared to control); C — control; n ≥ 8
Fig. 2. Effect of blockade of Panx1 channels by probenecid on NO secretion by astrocytes during their incubation with thrombin/LPS. C — control; * — р < 0.05 and # — р < 0.05 (compared to control), n ≥ 6
Fig. 3. Effect of Panx1 blockade on thrombin/LPS-induced HEX secretion by mouse astrocytes. HEX secretion was assessed 24 h after thrombin/LPS exposure. C — control; * — р < 0.05, # — р < 0.05 (compared with control), n ≥ 6
Fig. 4. Effect of Panx1 blockade on thrombin/LPS-induced HEX secretion by mouse astrocytes. HEX secretion was assessed 48 h after thrombin/LPS exposure. C — control; * р < 0.05, # р < 0.05 (compared to control), n ≥ 6
Fig. 5. Effect of Panx1 blockade on IL6 secretion by mouse astrocytes exposed to thrombin/LPS for 6 hours. C — control; * р < 0.05, # р < 0.05 (compared to control), n ≥ 3
Fig. 6. Effect of probenecid on thrombin/LPS-induced activation of astrocytes