ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2024 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Effect of probenecid on astrocyte activation in vitro
About authors
1 Pirogov Russian National Research Medical University, Moscow, Russia
2 Lomonosov Moscow State University, Moscow, Russia
Correspondence should be addressed: Lyubov R. Gorbacheva
Ostrovitianova, 1, Moscow, 117997, Russia; ur.liam@76ibrog
About paper
Funding: The study was supported by the Russian Science Foundation, Project № 22-25-00848.
Author contribution: Babkina II, Morozova MP — obtaining and maintaining primary culture of astrocytes; collection, interpretation and statistical analysis of data, manuscript writing; Mazeeva VV — obtaining and maintaining primary culture of astrocytes, interpretation and statistical analysis of data; Gorbacheva LR — conception and design of the experiment, interpretation of data, project management, manuscript writing.
Compliance with ethical standards: the study was approved by the Ethical Committee of Pirogov Russian National Research Medical University (Protocol № 23/2021 dated December 13, 2021).
Received: 2023-12-13
Accepted: 2024-01-24
Published online: 2024-02-26
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Fig. 1. Effect of Panx1 blockade on survival of thrombin- or LPS-activated astrocytes. * — р < 0.05, # — р < 0.05 (compared to control); C — control; n ≥ 8
Fig. 2. Effect of blockade of Panx1 channels by probenecid on NO secretion by astrocytes during their incubation with thrombin/LPS. C — control; * — р < 0.05 and # — р < 0.05 (compared to control), n ≥ 6
Fig. 3. Effect of Panx1 blockade on thrombin/LPS-induced HEX secretion by mouse astrocytes. HEX secretion was assessed 24 h after thrombin/LPS exposure. C — control; * — р < 0.05, # — р < 0.05 (compared with control), n ≥ 6
Fig. 4. Effect of Panx1 blockade on thrombin/LPS-induced HEX secretion by mouse astrocytes. HEX secretion was assessed 48 h after thrombin/LPS exposure. C — control; * р < 0.05, # р < 0.05 (compared to control), n ≥ 6
Fig. 5. Effect of Panx1 blockade on IL6 secretion by mouse astrocytes exposed to thrombin/LPS for 6 hours. C — control; * р < 0.05, # р < 0.05 (compared to control), n ≥ 3
Fig. 6. Effect of probenecid on thrombin/LPS-induced activation of astrocytes
