2024 / 01

Next Paper
DOI: 10.24075/brsmu.2024.005
Copyright: © 2024 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).

ORIGINAL RESEARCH

Effect of probenecid on astrocyte activation in vitro

Babkina II1, Mazeeva VV2, Morozova MP1, Gorbacheva LR1,2
About authors

1 Pirogov Russian National Research Medical University, Moscow, Russia

2 Lomonosov Moscow State University, Moscow, Russia

Correspondence should be addressed: Lyubov R. Gorbacheva
Ostrovitianova, 1, Moscow, 117997, Russia; ur.liam@76ibrog

About paper

Funding: The study was supported by the Russian Science Foundation, Project № 22-25-00848.

Author contribution: Babkina II, Morozova MP — obtaining and maintaining primary culture of astrocytes; collection, interpretation and statistical analysis of data, manuscript writing; Mazeeva VV — obtaining and maintaining primary culture of astrocytes, interpretation and statistical analysis of data; Gorbacheva LR — conception and design of the experiment, interpretation of data, project management, manuscript writing.

Compliance with ethical standards: the study was approved by the Ethical Committee of Pirogov Russian National Research Medical University (Protocol № 23/2021 dated December 13, 2021).

Received: 2023-12-13 Accepted: 2024-01-24 Published online: 2024-02-26
|
  1. Choi SH, Da YL, Seung UK, Byung KJ. Thrombin-induced oxidative stress contributes to the death of hippocampal neurons in vivo: Role of microglial NADPH oxidase. Journal of Neuroscience. 2005; 25: 4082–90.
  2. D’hondt C, Ponsaerts R, De Smedt H, Vinken M, De Vuyst E, De Bock M, et al. Pannexin channels in ATP release and beyond: An unexpected rendezvous at the endoplasmic reticulum. Cellular Signalling. 2011; 23: 305–16.
  3. Koval M, Cwiek A, Carr T, Good ME, Lohman AW, Isakson BE. Pannexin 1 as a driver of inflammation and ischemia–reperfusion injury. Purinergic Signalling. 2021; 17: 521–31.
  4. Galkov MD, Surin AM, Lisina OYu, Gorbacheva LR. Neurodegeneration and Neuroinflammation: The Role of Pannexin 1. Neurochemical Journal. 2023; 17: 727–39.
  5. Rusiecka OM, Tournier M, Molica F, Kwak BR. Pannexin1 channels — a potential therapeutic target in inflammation. Front Cell Dev Biol. 2022; 10: 1020826.
  6. García-Rodríguez C, Mujica P, Illanes-González J, López A, Vargas C, Sáez JC, et al. Probenecid, an Old Drug with Potential New Uses for Central Nervous System Disorders and Neuroinflammation. Biomedicines. 2023; 11.
  7. Ivanova AE, Gorbacheva LR, Strukova SM, Pinelic VG, Rajzer G. Uchastie aktivirovannogo proteina S i trombina v reguljacii funkcij astrocitov. Biol membrany. 2013; 30: 387–97.
  8. Schwartz LB, Austen KF, Wasserman SI. Immunologic Release of β-Hexosaminidase and β-Glucuronidase from Purified Rat Serosal Mast Cells. The Journal of Immunology. 1979; 123: 1445–50.
  9. Penuela S, Gehi R, Laird DW. The biochemistry and function of pannexin channels. Biochimica et Biophysica Acta — Biomembranes. 2013; 1828: 15–22.
  10. Bhaskaracharya A, Dao-Ung P, Jalilian I, Spildrejorde M, Skarratt KK, Fuller SJ, et al. Probenecid Blocks Human P2X7 ReceptorInduced Dye Uptake via a Pannexin-1 Independent Mechanism. PLoS ONE. 2014; 9: e93058.
  11. Locovei S, Wang J, Dahl G. Activation of pannexin 1 channels by ATP through P2Y receptors and by cytoplasmic calcium. FEBS Letters. 2006; 580: 239–44.
  12. Yang K, Xiao Z, He X, Weng R, Zhao X, Sun T. Mechanisms of Pannexin 1 (PANX1) Channel Mechanosensitivity and Its Pathological Roles. International Journal of Molecular Sciences. 2022; 23.
  13. Szydlowska K, Tymianski M. Calcium, ischemia and excitotoxicity. Cell Calcium. 2010; 47: 122–9.
  14. Orellana JA, Froger N, Ezan P, Jiang JX, Bennett MVL, Naus CC, et al. ATP and glutamate released via astroglial connexin 43 hemichannels mediate neuronal death through activation of pannexin 1 hemichannels. Journal of Neurochemistry. 2011; 118: 826–40.
  15. Gödecke S, Roderigo C, Rose CR, Rauch BH, Gödecke A, Schrader J. Thrombin-induced ATP release from human umbilical vein endothelial cells. Am J Physiol Cell Physiol. 2012; 302: 915–23.
  16. Chen X, Zhang H, Hao H, Zhang X, Song H, He B, et al. Thrombin induces morphological and inflammatory astrocytic responses via activation of PAR1 receptor. Cell Death Discovery. 2022; 8.
  17. Seminario-Vidal L, Kreda S, Jones L, O’Neal W, Trejo JA, Boucher RC, et al. Thrombin promotes release of ATP from lung epithelial cells through coordinated activation of Rho- and Ca2+ -dependent signaling pathways. Journal of Biological Chemistry. 2009; 284: 20638–48.
  18. Fiebich BL, Batista CRA, Saliba SW, Yousif NM, de Oliveira ACP. Role of microglia TLRs in neurodegeneration. Frontiers in Cellular Neuroscience. 2018; 12.
  19. Zhang Z, Lei Y, Yan C, Mei X, Jiang T, Ma Z, et al. Probenecid Relieves Cerebral Dysfunction of Sepsis by Inhibiting Pannexin 1-Dependent ATP Release. Inflammation. 2019; 42: 1082–92.
  20. Yu D-K, Lee B, Kwon M, Yoon N, Shin T, Kim N-G, et al. Phlorofucofuroeckol B suppresses inflammatory responses by down-regulating nuclear factor κB activation via Akt, ERK, and JNK in LPS-stimulated microglial cells. International Immunopharmacology. 2015; 28: 1068–75.
  21. Kozuka N, Itofusa R, Kudo Y, Morita M. Lipopolysaccharide and proinflammatory cytokines require different astrocyte states to induce nitric oxide production. J of Neuroscience Research. 2005; 82: 717–28.
  22. Chen Q, Liang Z, Yue Q, Wang X, Siu SWI, Pui-Man Hoi M, et al. A Neuropeptide Y/F-like Polypeptide Derived from the Transcriptome of Turbinaria peltata Suppresses LPS-Induced Astrocytic Inflammation. J Nat Prod. 2022; 85: 1569–80.
  23. Liu B, Gao H-M, Wang J-Y, Jeohn G-H, Cooper CL, Hong J-S. Role of nitric oxide in inflammation-mediated neurodegeneration. Ann N Y Acad Sci. 2002; 962: 318–31.
  24. Li T, Xu T, Zhao J, Gao H, Xie W. Depletion of iNOS-positive inflammatory cells decelerates neuronal degeneration and alleviates cerebral ischemic damage by suppressing the inflammatory response. Free Radic Biol Med. 2022; 181: 209–20.
  25. Boven LA, Vergnolle N, Henry SD, Silva C, Imai Y, Holden J, et al. Up-Regulation of Proteinase-Activated Receptor 1 Expression in Astrocytes During HIV Encephalitis. The Journal of Immunology. 2003; 170: 2638–46.
  26. Isakson BE, Thompson RJ. Pannexin-1 as a potentiator of ligandgated receptor signaling. Channels. 2014; 8: 118–23.
  27. Huang G, Bao J, Shao X, Zhou W, Wu B, Ni Z, et al. Inhibiting pannexin-1 alleviates sepsis-induced acute kidney injury via decreasing NLRP3 inflammasome activation and cell apoptosis. Life Sciences. 2020; 254.
  28. Wareham KJ, Seward EP. P2X7 receptors induce degranulation in human mast cells. Purinergic Signalling. 2016; 12: 235–46.
  29. Ponath G, Park C, Pitt D. The role of astrocytes in multiple sclerosis. Frontiers in Immunology. 2018; 9.
  30. Okada S, Nakamura M, Mikami Y, Shimazaki T, Mihara M, Ohsugi Y, et al. Blockade of interleukin-6 receptor suppresses reactive astrogliosis and ameliorates functional recovery in experimental spinal cord injury. Journal of Neuroscience Research. 2004; 76 (2): 265–76.
  31. Krasovska V, Doering LC. Regulation of IL6 secretion by astrocytes via TLR4 in the fragile X mouse model. Frontiers in Molecular Neuroscience. 2018; 11: 272.
  32. Wei L, Sheng H, Chen L, Hao B, Shi X, Chen Y. Effect of pannexin-1 on the release of glutamate and cytokines in astrocytes. Journal of Clinical Neuroscience. 2016; 23: 135–41.
  33. Shieh CH, Heinrich A, Serchov T, van Calker D, Biber K. P2X7dependent, but differentially regulated release of IL6, CCL2, and TNF-α in cultured mouse microglia. GLIA. 2014; 62: 592–607.
  34. Jian Z, Ding S, Deng H, Wang J, Yi W, Wang L, et al. Probenecid protects against oxygen–glucose deprivation injury in primary astrocytes by regulating inflammasome activity. Brain Research. 2016; 1643: 123–9.
  35. Hainz N, Wolf S, Tschernig T, Meier C. Probenecid Application Prevents Clinical Symptoms and Inflammation in Experimental Autoimmune Encephalomyelitis. Inflammation. 2016; 39: 123–8.
  36. Wang Q, Li H, Ling Z, Chen G, Wei Z-Y. Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses. J Neuroinflammation. 2022; 19: 244.