ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2024 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Information capacity of the NF-κB and AP-1 signaling activation sensors in in vitro assessment of dermatotoxic effects
About authors
Institute of Biomedical Chemistry, Moscow, Russia
Correspondence should be addressed: Tatiana V. Tolstova
Pogodinskaya, 10, s. 8, Moscow, 119121, Russia; ur.ksm.cmbi@avotslot
About paper
Funding: the study was performed as part of the Programme for Fundamental Scientific Research in the Russian Federation for the long-term period (2021–2030) (№ 122022800481-0).
Author contribution: Tolstova TV — experimental procedure, data acquisition, analysis, and interpretation, manuscript writing, illustrations; Pureczcky VK — experimental procedure, illustrations; Kozhin PM — literature review, manuscript writing, illustrations; Luzgina NG — study concept and design, manuscript writing; Rusanov AL — study planning, manuscript writing, overall guidance for the study.
Received: 2023-12-11
Accepted: 2024-02-16
Published online: 2024-02-27
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Fig. 1. Fluorescence intensity of transduced cells: control cells (А, C) and transduced cells showing high fluorescence intensity (B, D) in response to stimulation with 20 ng/mL TNFα for HaCaT/NF-κB (gate Р1) and 10 µМ Cd(NO3)2 for HaCaT/AP-1 (gate Р2) throughout 24 h
Fig. 2. Changes in the HaCaT/NF-κB cells’ relative fluorescence intensity under exposure to TNFα (А, B) and LPS (C, D). Fluorometry results (А, C) and fluorescence microscopy (B, D). * — significant differences from the previously reported concentration, p < 0.05.
Fig. 3. Assessing activation of the genes encoding the NF-κB transcription factor subunits. Relative expression of RelA and NFKB1 mRNA under exposure to TNFα (А, B) and LPS (C, D). * — significant differences from the control group, p < 0.05
Fig. 4. Assessing activation of the gene encoding the AP-1 transcription factor subunit. Relative expression of the C-JUN mRNA under exposure to Cd(NO3)2 (А) and UVA (B). * — significant differences from the control group, p < 0.05
Table 1. Sequences of PCR primers
Table 2. Changes in the HaCaT/AP-1 cell fluorescence associated with exposure to Cd(NO3)2
Note: * — significant differences from the control group, p < 0.05
