ORIGINAL RESEARCH
Information capacity of the NF-κB and AP-1 signaling activation sensors in in vitro assessment of dermatotoxic effects
Institute of Biomedical Chemistry, Moscow, Russia
Correspondence should be addressed: Tatiana V. Tolstova
Pogodinskaya, 10, s. 8, Moscow, 119121, Russia; ur.ksm.cmbi@avotslot
Funding: the study was performed as part of the Programme for Fundamental Scientific Research in the Russian Federation for the long-term period (2021–2030) (№ 122022800481-0).
Author contribution: Tolstova TV — experimental procedure, data acquisition, analysis, and interpretation, manuscript writing, illustrations; Pureczcky VK — experimental procedure, illustrations; Kozhin PM — literature review, manuscript writing, illustrations; Luzgina NG — study concept and design, manuscript writing; Rusanov AL — study planning, manuscript writing, overall guidance for the study.
- OECD, Test No. 429: Skin Sensitisation: Local Lymph Node Assay. Paris: Organisation for Economic Co-operation and Development, 2010. Accessed: Dec. 07, 2023. Available from: https://www.oecd-ilibrary.org/environment/test-no-429-skinsensitisation_9789264071100-en.
- OECD, Test No. 406: Skin Sensitisation. Paris: Organisation for Economic Co-operation and Development, 2022. Accessed: Dec. 06, 2023. Available from: https://www.oecd-ilibrary.org/environment/test-no-406-skin-sensitisation_9789264070660-en.
- Metody ispytanija po vozdejstviju himicheskoj produkcii na organizm cheloveka. Ispytanija po ocenke kozhnoj sensibilizacii: GOST 32375-2013. Vved. 01-08-2014. M.: Izd-vo standartov, 2014. Russian.
- Metody ispytanija po vozdejstviju himicheskoj produkcii na organizm cheloveka. Opredelenie toksichnosti pri povtornom/ mnogokratnom nakozhnom postuplenii. 28/21-dnevnyj test. GOST 32642-2014. Vved. 01-08-2014. M.: Izd-vo standartov, 2014. Russian.
- Caloni F, De Angelis I, Hartung T. Replacement of animal testing by integrated approaches to testing and assessment (IATA): a call for in vivitrosi. Arch Toxicol. 2022; 96 (7): 1935–1950. DOI: 10.1007/s00204-022-03299-x.
- Test Methods, PETA Science Consortium International e.V. Accessed: Jul. 25, 2023. Available from: https://www.thepsci.eu/test-methods/.
- Bergmann MM, Caubet J-C. Role of in vivo and in vitro Tests in the Diagnosis of Severe Cutaneous Adverse Reactions (SCAR) to Drug. Curr Pharm Des. 2019; 25 (36): 3872–80. DOI: 10.2174/1381612825666191107104126.
- OECD, Test No. 492: Reconstructed human Cornea-like Epithelium (RhCE) test method for identifying chemicals not requiring classification and labelling for eye irritation or serious eye damage. Paris: Organization for Economic Co-operation and Development, 2023. Accessed: Dec. 06, 2023. Available from: https://www.oecd-ilibrary.org/environment/test-no-492-reconstructed-human-cornea-like-epithelium-rhce-test-method-for-identifying-chemicals-not-requiring-classification-and-labelling-for-eye-irritation-or-serious-eye-damage_9789264242548-en.
- Metody ispytanij po vozdejstviju himicheskoj produkcii na organizm cheloveka. Metody ispytanij s primeneniem rekonstruirovannogo rogovogo jepitelija cheloveka (RhCE) dlja opredelenija himicheskoj produkcii, ne trebujushhej klassifikacii opasnosti kak vyzyvajushhej razdrazhenie ili ser'eznoe povrezhdenie glaz. GOST 34735-2021. Vved. 01.05.2024. M.: Izd-vo standartov, 2021. Russian.
- OECD, Test No. 431: In vitro skin corrosion: reconstructed human epidermis (RHE) test method. Paris: Organisation for Economic Co-operation and Development, 2019. Accessed: Dec. 06, 2023. Available from: https://www.oecd-ilibrary.org/environment/test-no-431-in-vitro-skin-corrosion-reconstructed-human-epidermis-rhe-test-method_9789264264618-en.
- Metody ispytanij po vozdejstviju himicheskoj produkcii na organizm cheloveka. Raz"edanie kozhi in vitro. Metody s ispol'zovaniem rekonstruirovannogo chelovecheskogo jepidermisa. GOST 32634-2020. Vved. 01.07.2021. M.: Standartinform, 2021. Russian.
- Rusanov AL, Luzgina NG, Lisica AV. Citotoksichnost' dodecilsul'fata natrija v otnoshenii keratinocitov linii HaCaT: sravnitel'nyj analiz razlichnyh metodov ocenki zhiznesposobnosti kletok. Bjulleten' jeksperimental'noj biologii i mediciny. 2017; 163 (2): 256–60. Russian.
- Arslanbaeva LR, Zherdeva VV, Ivashina TV, Vinokurov LM, Rusanov AL, Savickij AP. Geneticheski kodiruemaja Fret-para na osnove terbijsvjazyvajushhego peptida i krasnogo fluorescentnogo belka. Prikladnaja biohimija i mikrobiologija. 2010; 46 (2): 166–71. Russian.
- Rusanov AL, Savitsky AP. Fluorescence resonance energy transfer between fluorescent proteins as powerful toolkits for in vivo studies. Laser Physics Letters. 2011; 8 (2): 91–102. DOI: 10.1002/lapl.201010107.
- Rusanov AL, Luzgina ED, Vahrushev IV, Nahod KV, Luzgina NG. Kletochnaja model' stenki tonkogo kishechnika cheloveka na osnove geneticheski modificirovannyh kletok linii Saco-2. Kletochnye tehnologii v biologii i medicine. 2018; 3: 201–4. Russian.
- Emter R, van der Veen JW, Adamson G, Ezendam J, van Loveren H, Natsch A. Gene expression changes induced by skin sensitizers in the KeratinoSensTM cell line: Discriminating Nrf2dependent and Nrf2-independent events. Toxicol In Vitro. 2013; 27 (8): 2225–32. DOI: 10.1016/j.tiv.2013.09.009.
- Guo H, Ji J, Sun J, Zhang Y, Sun X. Development of a living mammalian cell-based biosensor for the monitoring and evaluation of synergetic toxicity of cadmium and deoxynivalenol. Science of the Total Environment. 2021; 771: 144823. DOI: 10.1016/j.scitotenv.2020.144823.
- Shen Q, et al. Adipocyte reporter assays: Application for identification of anti-inflammatory and antioxidant properties of mangostin xanthones. Mol Nutr Food Res. 2014; 58 (2): 239–47. DOI: 10.1002/mnfr.201300181.
- Buchanan V. H.R.2565 — 117th Congress (2021-2022): FDA Modernization Act of 2021. Accessed: Jul. 25, 2023. Available from: http://www.congress.gov/bill/117th-congress/house-bill/2565.
- Deyrieux AF, Wilson VG. In vitro culture conditions to study keratinocyte differentiation using the HaCaT cell line. Cytotechnology. 2007; 54 (2): 77–83. DOI: 10.1007/s10616-007-9076-1.
- Micallef L, et al. Effects of extracellular calcium on the growthdifferentiation switch in immortalized keratinocyte HaCaT cells compared with normal human keratinocytes. Exp Dermatol. 2009; 18 (2): 143–51. DOI: 10.1111/j.1600-0625.2008.00775.x.
- Rusanov AL, et al. Impact of p53 Knockout on Protein Data Set of HaCaT Cells in Confluent and Subconfluent Conditions. Data. 2022; 7 (3). DOI: 10.3390/data7030027.
- Rusanov AL, Romashin DD, Zgoda VG, Butkova TV, Luzgina NG. Protein dataset of immortalized keratinocyte HaCaT cells and normal human keratinocytes. Data in Brief. 2021; 35: 106871. DOI: 10.1016/j.dib.2021.106871.
- Sergachev I, Rusanov A, Trushkin E, Sakharov D, Marx U, Tonevitsky A. Fluorescent optical fiber sensors for cell viability monitoring. Analyst. 2013; 138 (14): 4066–9. DOI: 10.1039/c3an00248a.
- Forcina GC, Conlon M, Wells A, Cao JY, Dixon SJ. Systematic quantification of population cell death kinetics in mammalian cells. Cell Syst. 2017; 4 (6): 600–10. DOI: 10.1016/j.cels.2017.05.002.
- OECD, Test No. 442D: In Vitro Skin Sensitisation: ARE-Nrf2 Luciferase Test Method. Paris: Organisation for Economic Cooperation and Development, 2022. Accessed: Jul. 25, 2023. Available from: https://www.oecd-ilibrary.org/environment/test-no-442d-in-vitro-skin-sensitisation_9789264229822-en.