ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
The candidate anti-tuberculosis mRNA vaccine immunogenicity and reactogenicity dependency on the animal’s sex and the vaccine dose
About authors
1 Sirius University of Science and Technology, Sirius, Russia
2 Central Tuberculosis Research Institute, Moscow, Russia
3 National Research Centre "Kurchatov Institute", Moscow, Russia
Correspondence should be addressed: Vasily V. Reshetnikov
Olimpiysky prospekt, 1, Sochi, 354340, Russia; ur.hepsuitnalat@vv.vokintehser
About paper
Funding: the study was supported by the Ministry of Science and Higher Education of the Russian Federation (agreement No. 075-10-2021-113, project ID RF----193021X0001).
Acknowledgements: the authors express their gratitude to staff members of the Sirius University of Science and Technology: I. M. Terenin for in vitro transcription, O. V. Zaborova for formulation of mRNA into lipid nanoparticles.
Author contribution: Reshetnikov VV — mRNA vaccine preparation, experimental procedure, manuscript writing; Shepelkova GS — experimental procedure, data analysis; Rybakova AV — experimental procedure, data analysis; Trashkov AP — planning the experiment, manuscript editing; Yeremeev VV — planning the experiment, manuscript editing; Ivanov RA — planning the experiment, manuscript editing.
Compliance with ethical standards: the study was approved by the Ethics Committee of the National Research Centre "Kurchatov Institute" (protocol No. 2 dated 31 October 2023) and conducted in accordance with the Order of the Ministry of Health No. 755 and the Guidelines issued by the Office of Laboratory Animal Welfare (А5502-01).
Received: 2024-09-16
Accepted: 2024-10-04
Published online: 2024-10-30
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Fig. 1. mRNA vaccine immunogenicity assessment. А. Experimental design. B. ELISPOT analysis data for cells of the spleen and inguinal lymph nodes. IPC – IFNγ-producing cells. The data are provided as mean ± error of the mean. * — p < 0.05; * * — p < 0.01; * * * — p < 0.001 compared to appropriate control group (PBS); ### — p < 0.001 compared to the group administered 15 µg of RNA
Table 1. Hematological parameters of blood
Note: the data are presented as median and interquartile range (Q25–Q75). The data on the absolute lymphocyte and neutrophil counts are not provided, but significance of intergroup differences is the same when comparing absolute values. * — the difference from control values is significant at the selected significance level (p < 0.05). WBC (white blood cells) — white blood cell count; Neu% — relative neutrophil count; Lym% — relative lymphocyte count; Mon% — relative monocyte count; Eos% — relative eosinophil count; Bas% — relative basophil count; RBC (red blood cells) — absolute red blood cell count; HGB (hemoglobin) — hemoglobin concentration; HCT — hematocrit; PLT (platelets) — absolute platelet count.
Table 2. Biochemical parameters of blood
Note: the data are presented as median and interquartile range (Q25–Q75). * — the difference from control values is significant at the selected significance level (p < 0.05)
