Funding: the study was supported by the Ministry of Science and Higher Education of the Russian Federation (agreement No. 075-10-2021-113, project ID RF----193021X0001).

Acknowledgements: the authors express their gratitude to staff members of the Sirius University of Science and Technology: I. M. Terenin for in vitro transcription, O. V. Zaborova for formulation of mRNA into lipid nanoparticles.

Author contribution: Reshetnikov VV — mRNA vaccine preparation, experimental procedure, manuscript writing; Shepelkova GS — experimental procedure, data analysis; Rybakova AV — experimental procedure, data analysis; Trashkov AP — planning the experiment, manuscript editing; Yeremeev VV — planning the experiment, manuscript editing; Ivanov RA — planning the experiment, manuscript editing.

Compliance with ethical standards: the study was approved by the Ethics Committee of the National Research Centre "Kurchatov Institute" (protocol No. 2 dated 31 October 2023) and conducted in accordance with the Order of the Ministry of Health No. 755 and the Guidelines issued by the Office of Laboratory Animal Welfare (А5502-01).