ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2024 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Strains of Mycobacterium tuberculosis with mutations in gyrA differ in their level of competitive fitness
About authors
1 Central Tuberculosis Research Institute, Moscow, Russia
2 Yevdokimov Moscow State University of Medicine and Dentistry, Moscow, Russia
Correspondence should be addressed: Sophia Nikolayevna Andreevskaya
Yauzskaya Alley, 2 p. 1A, Moscow, 107564, Russia; ur.liam@aifosdna
About paper
Funding: the study was conducted under the State assignment of FGBNU ‘CNIIT’ No. NIOCTR 122041100246-3 ‘Interspecies and intraspecies polymorphism of mycobacteria in patients with tuberculosis and mycobacteriosis in the presence of specific therapy’.
Author contribution: Chernousova LN, Ergeshov A — development of the research design; Ustinova VV, Kiseleva EA — conducting the experiment; Smirnova TG — conducting the experiment, analyzing the obtained data; Larionova EE — analyzing the obtained data; Sevastyanova EV — review of publications on the topic of the research; Andreevskaya SN — analyzing the obtained data, writing the manuscript.
Received: 2024-11-05
Accepted: 2024-11-29
Published online: 2024-12-19
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Table 1. Characteristics of MTB strains
Note: the strain code was assigned based on the mutation in the gyrA gene (codon and amino acid substitution); WT — MTB strain with wild type gyrA; H — isoniazid; R — rifampicin; E — ethambutol; Z — pyrazinamide; Eth — ethionamide; Lfx — levofloxacin; SIT (Spoligotype International Type) — international code of the spoligotype as per the SITVIT2 database (http: //www.pasteur-guadeloupe.fr: 8081/SITVIT2/).
Table 2. Competitive fitness (W) of MTB strains with different genetic determinants of resistance to pharmaceutical chemicals, determined on the 21st day of the experiment
Note: signs < and > indicate which strain has less/more fitness when co-cultivated, and sign = shows that the level of competitive fitness of a pair of strains is similar
Table 3. Specific growth rate (µ) of MTB strains with different genetic determinants of resistance to pharmaceutical chemicals
Note: * — µ is more than 10% lower than in individual cultivation; * * — µ is more than 10% higher than in individual cultivation
