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ORIGINAL RESEARCH

Intranasal lipopolysaccharide administration to Sprague-Dawley rats as a biomodel of acute respiratory distress syndrome

Kiseleva VV1, Vishnyakova PA2, Kosyreva AM3, Kananykhina EYu3, Emelianov II1, Elchaninov AV3, Fatkhudinov TH3
About authors

1 Patrice Lumumba Peoples' Friendship University of Russia, Moscow, Russia

2 Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia

3 Petrovsky National Research Centre of Surgery, Moscow, Russia

Correspondence should be addressed: Victoria V. Kiseleva
Oparina, 4, Moscow, 117997, Russia; moc.liamg@1991.avosonruk.airotciv

About paper

Funding: the study was supported by the Russian Science Foundation (grant No. 24-25-00203).

Acknowledgements: the authors would like to thank D.A. Areshidze, C. Sci. Biol., Head of the Cellular Pathology Laboratory of the Avtsyn Institute of Human Morphology for performing the complete blood count test in animals.

Author contribution: Kiseleva VV — experimental design and procedure, analysis of the results, manuscript writing; Vishnyakova PA — advice on the experimental procedure, material resources, editing; Kosyreva AM — advice on the experimental procedure, editing; Kananykhina EYu, Emelianov II — animal handling; Elchaninov AV — advice on the experimental procedure, material resources, editing; Fatkhudinov TH — material resources for the study.

Compliance with ethical standards: the study was approved by the Ethics Committee of the Avtsyn Institute of Human Morphology (protocol No. 21 dated 29 March 2019). Animals were handled in accordance to the ARRIVE guidelines and the Directive ЕС 2010/63/EU on the protection of animals used for scientific purposes.

Received: 2025-04-21 Accepted: 2025-05-06 Published online: 2025-05-20
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  1. Bos LDJ, Ware LB. Acute respiratory distress syndrome: causes, pathophysiology, and phenotypes. The Lancet. 2022; 400: 1145–56.
  2. Aeffner F, Bolon B, Davis IC. Mouse Models of Acute Respiratory Distress Syndrome. Toxicol Pathol. 2015; 43: 1074–92.
  3. Matute-Bello G, Frevert CW, Martin TR. Animal models of acute lung injury. American Journal of Physiology-Lung Cellular and Molecular Physiology. 2008; 295: L379–99.
  4. Soloveva T, Davydova V, Krasikova I, Yermak I. Marine Compounds with Therapeutic Potential in Gram-Negative Sepsis. Mar Drugs. 2013; 11: 2216–29.
  5. Khadangi F, Forgues A-S, Tremblay-Pitre S, Dufour-Mailhot A, Henry C, Boucher M, et al. Intranasal versus intratracheal exposure to lipopolysaccharides in a murine model of acute respiratory distress syndrome. Sci Rep. 2021; 11: 7777.
  6. Kosyreva AM, Miroshnichenko EA, Tsvetkov IS, Lokhonina AV, Sentyabreva AV, Dzhalilova DSh, et al. Morphofunctional Characteristics of Lung Macrophages in Rats with Acute Respiratory Distress Syndrome. Bull Exp Biol Med. 2023; 175: 822–7.
  7. Available from: https://floreada.io/.
  8. Parker JC, Townsley MI. Evaluation of lung injury in rats and mice. American Journal of Physiology-Lung Cellular and Molecular Physiology. 2004; 286: L231–46.
  9. Vishnyakova P, Poltavets A, Karpulevich E, Maznina A, Vtorushina V, Mikhaleva L, et al. The response of two polar monocyte subsets to inflammation. Biomedicine & Pharmacotherapy. 2021; 139: 111614.
  10. Chistiakov DA, Killingsworth MC, Myasoedova VA, Orekhov AN, Bobryshev YV. CD68/macrosialin: not just a histochemical marker. Laboratory Investigation. 2017; 97: 4–13.
  11. Murray PJ, Allen JE, Biswas SK, Fisher EA, Gilroy DW, Goerdt S, et al. Macrophage Activation and Polarization: Nomenclature and Experimental Guidelines. Immunity. 2014;41:14–20.
  12. Qazi MR, Bogdanska J, Butenhoff JL, Nelson BD, DePierre JW, Abedi-Valugerdi M. High-dose, short-term exposure of mice to perfluorooctanesulfonate (PFOS) or perfluorooctanoate (PFOA) affects the number of circulating neutrophils differently, but enhances the inflammatory responses of macrophages to lipopolysaccharide (LPS) in a similar fashion. Toxicology. 2009; 262: 207–14.
  13. Chakraborty S, Zawieja SD, Wang W, Lee Y, Wang YJ, von der Weid P-Y, et al. Lipopolysaccharide modulates neutrophil recruitment and macrophage polarization on lymphatic vessels and impairs lymphatic function in rat mesentery. American Journal of Physiology-Heart and Circulatory Physiology. 2015; 309: H2042–57.
  14. Torres M, Casado G, Vigón L, Rodríguez-Mora S, Mateos E, Ramos-Martín F, et al. Changes in the immune response against SARS-CoV-2 in individuals with severe COVID-19 treated with high dose of vitamin D. Biomedicine & Pharmacotherapy. 2022; 150: 112965.
  15. Riyaz Tramboo S, Elkhalifa AME, Quibtiya S, Ali SI, Nazir Shah N, Taifa S, et al. The critical impacts of cytokine storms in respiratory disorders. Heliyon. 2024; 10: e29769.
  16. Hou F, Xiao K, Tang L, Xie L. Diversity of Macrophages in Lung Homeostasis and Diseases. Front Immunol. 2021; 12.
  17. Fakoya AO, Naeem A, Louisdon P, Histology. Alveolar Macrophages. Available from: https://www.ncbi.nlm.nih.gov/books/NBK513313/#:~:text=Alveolar%20macrophages%20produce%20anti%2Dinflammatory,processes%20and%20promote%20tissue%20repair.
  18. Morrell ED, Bhatraju PK, Mikacenic CR, Radella F, Manicone AM, Stapleton RD, et al. Alveolar Macrophage Transcriptional Programs Are Associated with Outcomes in Acute Respiratory Distress Syndrome. Am J Respir Crit Care Med. 2019; 200: 732–41.
  19. Yao Y, Jeyanathan M, Haddadi S, Barra NG, Vaseghi-Shanjani M, Damjanovic D, et al. Induction of Autonomous Memory Alveolar Macrophages Requires T Cell Help and Is Critical to Trained Immunity. Cell. 2018; 175: 1634–50.e17.
  20. Mahida RY, Scott A, Parekh D, Lugg ST, Hardy RS, Lavery GG, et al. Acute respiratory distress syndrome is associated with impaired alveolar macrophage efferocytosis. European Respiratory Journal. 2021; 58: 2100829.
  21. Couper KN, Blount DG, Riley EM. IL-10: The Master Regulator of Immunity to Infection. The Journal of Immunology. 2008; 180: 5771–7.
  22. Sun Z, Chen A, Fang H, Sun D, Huang M, Cheng E, et al. B cell-derived IL-10 promotes the resolution of lipopolysaccharide-induced acute lung injury. Cell Death Dis. 2023; 14: 418.