ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2025 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Age-related alterations in the immune system of aging mice
About authors
Sirius University of Science and Technology, Sirius Federal Territory, Krasnodarsky Krai, Russia
Correspondence should be addressed: Daniil V. Shevyrev
Olimpiysky prospekt, 1, Sochi, 354349, Russia; ur.liam@52liinad.rd
About paper
Funding: the study was supported by the Russian Science Foundation работа, project No. 24-15-20003 https://rscf.ru/project/24-15-20003/ (date of access: August 19, 2025).
Author contribution: Matveeva KS — experimental procedure, data processing, manuscript formatting, Shevyrev DV — experimental procedure, statistical analysis, manuscript reviewing.
Compliance with ethical standards: the study was approved by the Ethics Committee of the Sirius University of Science and Technology (protocol No. 7.1 dated 12 April 2024).
Received: 2025-08-27
Accepted: 2025-09-23
Published online: 2025-10-08
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Fig. 1. Gating strategies for lymphoid and myeloid populations in spleen and bone marrow samples from mice (A) and quantification of the proportion of SA-β-Gal-positive cells within each analyzed population (B). The histogram on the right shows SPiDER fluorescence intensity in the 488-530/30 nm detection channel for the FMO control (without fluorogenic substrate) and the positive control (Control+, without bafilomycin A1)
Fig. 2. Proportions of lymphoid and myeloid populations in spleen (A) and bone marrow (B) samples. Additionally, the frequency of senescent-like cells positive for the SA-β-Gal marker in spleen (C) and bone marrow (D) is shown. Abbreviations: DC — dendritic cells; GrC — granulocytes; Mon — monocytes; Mph — macrophages; DN – double-negative T cells. Group comparisons were performed usin the Mann–Whitney U-test. Data are presented as Me ± interquartile range (IQR); * — p < 0.05, * * — p < 0.01, * * * — p < 0.001
