2025 / 06

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DOI: 10.24075/brsmu.2025.072
Copyright: © 2025 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).

METHOD

Preparation and procedure for scanning electron microscopy examination of cells adhered to bioceramic surfaces

Danilko KV , Solntsev VA , Plotnitsky RV , Mikhailova AV , Bilyalov AR , Chugunov SS , Galautdinov MF , Akbashev VN , Akhatov ISh
About authors

Bashkir State Medical University, Ufa, Russia

For correspondence: Xenia V. Danilko
Lenin St., Bldg. 3, Ufa, 450008, Russia; ur.xednay@oklinad-esk

About paper

Funding: the work was supported by grant No. 23-15-20042 from the Russian Science Foundation. The study utilized the equipment purchased under the "Priority 2030" program.

Author contribution: Danilko KV — study planning, development of study protocols, analysis of results, conducting SEM analysis, manuscript drafting; Solntsev VA, Plotnitsky RV — cell culturing, preparation for SEM; Mikhailova AV — conducting SEM analysis, analysis of results; Bilyalov AR — study planning, literature analysis, interpretation of data, manuscript drafting; Chugunov SS — study planning, literature analysis; Galautdinov MF — data collection, analysis, interpretation; Akbashev VN — literature analysis, manuscript drafting; Akhatov IS — study planning, manuscript editing.

Received: 2025-11-13 Accepted: 2025-12-04 Published online: 2025-12-17
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  1. Zhang Y, Shen Y, Peng F, Jiang C, Li W, Miao H, Huang F, Zeng Y. Method for acquiring accurate coordinates of the source point in electron backscatter diffraction. J Microsc. 2021; 284 (3): 233– 243. DOI: 10.1111/jmi.13055. PubMed PMID: 34383320.
  2. Eliaz N, Metoki N. Calcium Phosphate Bioceramics: A Review of Their History, Structure, Properties, Coating Technologies and Biomedical Applications. Materials (Basel). 2017; 10 (4): 334. DOI: 10.3390/ma10040334. PubMed PMID: 28772697.
  3. Kim SE, Park K. Recent Advances of Biphasic Calcium Phosphate Bioceramics for Bone Tissue Regeneration. Adv Exp Med Biol. 2020; 1250: 177–88. DOI: 10.1007/978-981-15-3262-7_12. PubMed PMID: 32601945.
  4. Koga D, Kusumi S, Shibata M, Watanabe T. Applications of Scanning Electron Microscopy Using Secondary and Backscattered Electron Signals in Neural Structure. Front Neuroanat. 2021; 15: 759804. DOI: 10.3389/fnana.2021.759804. PubMed PMID: 34955763; PMCID: PMC8693767.
  5. Qu Y, Pan H, Peng R, Niu J, Li C. Interference illumination of three nonzero-order beams for LCOS-based structured illumination microscopy. J Microsc. 2019; 275 (2): 97–106. DOI: 10.1111/jmi.12806.. PubMed PMID: 31087655.
  6. Bray DF, Bagu J, Koegler P. Comparison of hexamethyldisilazane (HMDS), Peldri II, and critical-point drying methods for scanning electron microscopy of biological specimens. Microsc Res Tech. 1993; 26 (6): 489–95. DOI: 10.1002/jemt.1070260603. PubMed PMID: 8305726.
  7. Fakhrullin R, Nigamatzyanova L, Fakhrullina G. Dark-field/ hyperspectral microscopy for detecting nanoscale particles in environmental nanotoxicology research. Sci Total Environ. 2021; 772: 145478. DOI: 10.1016/j.scitotenv.2021.145478. PubMed PMID: 33571774.
  8. Orhan EO, Bahadır D, Akgün C, Aktaş S. Scanning electron microscopic evaluation of the efficacy of 5% ethylenediaminetetraacetic acid for smear layer removal. Microsc Res Tech. 2021; 84 (2): 253–60. DOI: 10.1002/jemt.23582. PMID: 32905662.
  9. Teixeira S, Ferraz MP, Monteiro FJ. Biocompatibility of highly macroporous ceramic scaffolds: cell adhesion and morphology studies. J Mater Sci Mater Med. 2008; 19 (2): 855–9. DOI: 10.1007/s10856-007-3005-x. Epub 2007 Aug 1. PubMed PMID: 17665126.
  10. Bilyalov A, Piatnitskaia S, Rafikova G, et al. The effect of sterilization methods on the cytotoxicity of ceramic medical implants. Bulletin of Russian State Medical University. 2025; 1: 77–84. DOI: 10.24075/brsmu.2025.009.
  11. Ullah N, Guhar D, Khan S. Preparation and topographical studies of various biological specimens using alternate method to critical point drying: Scanning electron microscopy. J Microsc. 2025; 299 (1): 25–35. DOI: 10.1111/jmi.13412. PubMed PMID: 40171741.
  12. Skliarenko Y, Kolomiiets VV, Balatskyi VV, et al. The impact of the physicochemical properties of calcium phosphate ceramics on biocompatibility and osteogenic differentiation of mesenchymal stem cells. BMC Res Notes. 2024; 17, 295. DOI: 10.1186/s13104-024-06937-y.
  13. Parisi L, Toffoli A, Ghezzi B, Lagonegro P, Trevisi G, et al. Preparation of hybrid samples for scanning electron microscopy (SEM) coupled to focused ion beam (FIB) analysis: A new way to study cell adhesion to titanium implant surfaces. PLOS ONE. 2022; 17 (8): e0272486. DOI: 10.1371/journal.pone.0272486.
  14. Gou Y, Qi K, Wei Y, Gu Z, Xie H. Advances of calcium phosphate nanoceramics for the osteoinductive potential and mechanistic pathways in maxillofacial bone defect repair. Nano TransMed. 2024; 3: 100033. DOI: 10.1016/j.ntm.2024.100033.