ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2025 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Comparative imaging of the HAEE-CY5 and ЕЕАН-СУ5 tetrapeptide binding to the Aβ aggregates on the SH-SY5Y cells
About authors
1 Research Institute of Translational Medicine, Pirogov Russian National Research Medical University, Moscow, Russia
2 Institute of Biomedical Engineering, MISIS University of Science and Technology, Moscow, Russia
Correspondence should be addressed: Anna V. Ivanova
Ostrovityanova, 1, str.1, Moscow, 117513, Russia; ur.xednay@tirofsof.repus
About paper
Funding: the work was carried out under the State Assignment "Development of a radiopharmaceutical for the diagnosis of Alzheimer's disease using the HAEE tetrapeptide as a vector molecule", EGISU R&D registration number 1024110600012-8-3.2.25;3.2.26;3.2.12.
Author contribution: Ivanova AV — literature review, manuscript writing; Chmelyuk NS — fixation, cell sample preparation for microscopic imaging, image acquisition and analysis using a confocal microscope; Kuzmichev IA — synthesis of Ac-HAEEGGGGK(ε-Cy5)-NH2 and Ac-EEAHGGGGK(ε-Cy5)-NH2 fluorescent peptides; Shilyaeva MI — cell sample preparation; Abakumov MA — goal setting, developing the study design, manuscript writing; all the authors contributed to preparation of the paper equally, they confirmed compliance of their authorship with the international ICMJE criteria.
Received: 2025-10-15
Accepted: 2025-11-13
Published online: 2025-11-24
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Fig. 1. Chemical structure of the test conjugated peptides
Fig. 2. Control of immunofluorescence staining specificity in the SH-SY5Y cells. Alignment of channels demonstrates localization of the alexa 488-stained target antigens (green) on the Aβ aggregates. The nuclei are DAPI stained (blue). Scale bar 20 µm
Fig. 3. Localization of the EEАН-Cy5 and НАЕЕ-Cy5 compounds in the SH-SY5Y cells. Signal of the Cy5-labeled compounds (red). The nuclei are DAPI stained (blue). Scale bar 20 µm
Fig. 4. Visualization of the НАЕЕ-Cy5 and ЕЕАН-Cy5 binding to the Aβ aggregates in the SH-SY5Y cells. Signal of the Cy5-labeled tetrapeptides (red). The alexa 488-labeled antibodies on Aβ (green). Yellow on the combined image indicates colocalization of peptides with Aβ aggregates. The nuclei are DAPI stained (blue). Scale bar 20 µm
