2026 / 03

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DOI: 10.24075/brsmu.2026.037
Copyright: © 2026 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).

ORIGINAL RESEARCH

Assessment of lipid profile stability in postmortem samples for the development of intraoperative diagnostic methods

Parochkina EV1 , Rumiantseva AA1 , Semenova DK1 , Bormotov DS1 , Temnov AA1,2 , Stupnikova GS1 , Zavorotnyuk DS1 , Bocharov KV1,3
About authors

1 Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Russia

2 Burnasyan Federal Medical Biophysical Center, Moscow, Russia

3 V. L. Talrose Institute for Energy Problems of Chemical Physics, Moscow, Russia

Correspondence should be addressed: Ekaterina V. Parochkina
Pervomaiskaya, 5, Dolgoprudny, 141701, Russia; ude.hcetsyhp@ve.aniramahs

About paper

Funding: the study was supported by the Russian Science Foundation within the framework of research project No. 23-69-10035.

Author contribution: Parochkina EV — study conceptualization, experiment, data analysis, article authoring; Rumiantseva AA, Semyonova DK, Stupnikova GS — experiment; Bormotov DS — experiment, data analysis; Temnov AA — study conceptualization, experiment; Zavorotnyuk DS — data analysis; Bocharov KV — fundraising, research management.

Compliance with ethical standards: the study was approved by the Commission for the Control of the maintenance and use of laboratory animals of the Preclinical Research Center of the Central Research Laboratory, Siberian State Medical University of the Ministry of Health of the Russian Federation (Minutes No. 1 of October 13, 2023). The study complied with Federal Law No. 61-FZ "On the Circulation of Medicines" and the 2023 Eurasian Economic Commission Board Guidelines for Working with Laboratory (Experimental) Animals During Preclinical (Non-clinical) Studies. The sanitary and epidemiological norms stipulated in SanPiN 3.3686-21 "Sanitary and epidemiological requirements for the prevention of infectious diseases" have been observed.

Received: 2026-05-12 Accepted: 2026-06-17 Published online: 2026-06-30
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  1. Pekov SI, Bormotov DS, Bocharova SI, Sorokin AA, Derkach MM, Popov IA. Mass spectrometry for neurosurgery: Intraoperative support in decision-making. Mass Spectrom Rev. 2025; 44 (1): 62–73.
  2. Cakmakci D, Karakaslar EO, Ruhland E, Chenard MP, Proust F, Piotto M, et al. Machine learning assisted intraoperative assessment of brain tumor margins using HRMAS NMR spectroscopy. PLoS Comput Biol. 2020; 16 (11): e1008184.
  3. Muthu M, Nordström A. Current Status and Future Prospects of Clinically Exploiting Cancer-specific Metabolism — Why Is Tumor Metabolism Not More Extensively Translated into Clinical Targets and Biomarkers? Int J Mol Sci. 2019; 20 (6): 1385.
  4. McCullumsmith RE, Meador-Woodruff JH. Novel Approaches to the Study of Postmortem Brain in Psychiatric Illness: Old Limitations and New Challenges. Biol Psychiatry. 2011; 69 (2): 127–33.
  5. Iacobuzio-Donahue CA, Michael C, Baez P, Kappagantula R, Hooper JE, Hollman TJ. Cancer biology as revealed by the research autopsy. Nat Rev Cancer. 2019; 19 (12): 686–97.
  6. Olney KC, Gibson KA, Cadiz MP, Rahimzadeh N, Swarup V, Fryer JD. Postmortem Interval Leads to Loss of Disease-Specific Signatures in Brain Tissue. eNeuro. 2025; 12 (3): ENEURO.0505-24.2025.
  7. Carithers LJ, Ardlie K, Barcus M, Branton PA, Britton A, Buia SA, et al. A Novel Approach to High-Quality Postmortem Tissue Procurement: The GTEx Project. Biopreserv Biobank. 2015; 13 (5): 311–9.
  8. Pozhitkov AE, Noble PA. Gene expression in the twilight of death. BioEssays. 2017; 39 (9): 1700066.
  9. Zhu Y, Wang L, Yin Y, Yang E. Systematic analysis of gene expression patterns associated with postmortem interval in human tissues. Sci Rep. 2017; 7 (1): 5435.
  10. Pozhitkov AE, Neme R, Domazet-Lošo T, Leroux BG, Soni S, Tautz D et al. Tracing the dynamics of gene transcripts after organismal death. Open Biol. 2017; 39 (9): e160267.
  11. Buyko EE, Perina EA, Sobakin DS, Tsyganov MM, Vasilchenko DV, Vtorushin SV, et al. Dynamics of Postmortem Gene Expression in Normal and Neoplastic Murine Liver. Life. 2026; 16 (4): 683.
  12. Parker GJ, McKiernan HE, Legg KM, Goecker ZC. Forensic proteomics. Forensic Sci Int Genet. 2021; 54: 102529.
  13. Zelentsova EA, Yanshole LV, Melnikov AD, Kudryavtsev IS, Novoselov VP, Tsentalovich YP. Post-mortem changes in metabolomic profiles of human serum, aqueous humor and vitreous humor. Metabolomics. 2020; 16 (7): 80.
  14. Wei J, Wong LC, Boland S. Lipids as Emerging Biomarkers in Neurodegenerative Diseases. Int J Mol Sci. 2023; 25 (1): 131.
  15. Paul B, Lewinska M, Andersen JB. Lipid alterations in chronic liver disease and liver cancer. JHEP Rep. 2022; 24 (6): e100479.
  16. Pekov SI, Sorokin AA, Kuzin AA, Bocharov KV, Bormotov DS, Shivalin AS et al. Analysis of phosphatidylcholines alterations in human glioblastomas ex vivo. Biochem Mosc Suppl B Biomed Chem. 2021; 15 (3): 241–7.
  17. Bonicelli A, Mickleburgh HL, Chighine A, Locci E, Wescott DJ, Procopio N. The ‘ForensOMICS’ approach for postmortem interval estimation from human bone by integrating metabolomics, lipidomics, and proteomics. Elife. 2022; 30 (11): e83658.
  18. Nolan AN, Mead RJ, Maker G, Speers SJ. A review of the biochemical products produced during mammalian decomposition with the purpose of determining the post-mortem interval. Aust J Forensic Sci. 2019; 52 (4): 477–88.
  19. Caballero-Moreno L, Luna A, Legaz I. Lipidomes in Cadaveric Decomposition and Determination of the Postmortem Interval: A Systematic Review. Int J Mol Sci. 2014; 25 (2): 984.
  20. Zavolskova M, Senko D, Bukato O, Troshin S, Stekolshchikova E, Kachanovski M, et al. Postmortem Stability Analysis of Lipids and Polar Metabolites in Human, Rat, and Mouse Brains. Biomolecules. 2025; 15 (9): e1288.
  21. Pekov SI, Zhvansky ES, Eliferov VA, Sorokin AA, Ivanov DG, Nikolaev EN, et al. Determination of Brain Tissue Samples Storage Conditions for Reproducible Intraoperative Lipid Profiling. Molecules. 2022; 27 (8): 2587.
  22. Bormotov DS, Eliferov VA, Peregudova OV, Zavorotnyuk DS, Bocharov KV, Pekov SI et al. Incorporation of a Disposable ESI Emitter into Inline Cartridge Extraction Mass Spectrometry Improves Throughput and Spectra Stability. J Am Soc Mass Spectrom. 2023; 34 (1): 119–22.
  23. Surma MA, Gerl MJ, Herzog R, Helppi J, Simons K, Klose C. Mouse lipidomics reveals inherent flexibility of a mammalian lipidome. Sci Rep. 2021; 11 (1): 19364.
  24. Buyko EE, Perina EA, Vasilchenko DV, Tsydenova IA, Khmelevskaya ES, Ufandeev AA, et al. Dynamic changes in RNA integrity, gene expression, and tissue pathomorphology of experimental mice in the postmortem period. Bull Sib Med. 2024; 23 (4): 5–14.
  25. Khan SA, Ilies MA. The Phospholipase A2 Superfamily: Structure, Isozymes, Catalysis, Physiologic and Pathologic Roles. Int J Mol Sci. 2023; 24 (2): 1353.
  26. Richmond GS, Smith TK. Phospholipases A1. Int J Mol Sci. 2011; 12 (1): 588–612.
  27. Ferreira PG, Muñoz-Aguirre M, Reverter F, Sá Godinho CP, Sousa A, Amadoz A, et al. The effects of death and post-mortem cold ischemia on human tissue transcriptomes. Nat Commun. 2018; 9 (1): 490.