ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2026 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
The effect of diclofenac sodium on bone marrow cellularity, the LSK cell pool, and cytokine profiles in mice with experimental dermatitis
About authors
1 MEDSI Clinic, Clinical Hospital No. 1, Moscow, Russia
2 Polyclinic on Smolenskaya Scientific and Diagnostic Center, Moscow, Russia
3 Saint Luka LSMU, Russia
Correspondence should be addressed: Alexey S. Ivanov
A. Dikogo, 16A, kv. 73, 111396, Moscow; ur.liam@5891golotamvartahsas
About paper
Acknowledgements: The authors express their sincere gratitude to S. Gramatyuk and M. Noebauer (UAB Global) for methodological support and significant contribution to the design, planning, and conceptualization of this experimental research.
Author contribution: Bondorenko DV, Ivanov AS — study design, experimental part of the work; Tananakina TP — study design, experimental part of the work, scientific support; Kashchenko SA — scientific text editing, scientific support, experiment preparation; Pogorelova IA — text editing, experiment preparation, animal care consultations, drug dose calculations; Alexandrov IA — animal care, experimental part of the work.
Compliance with ethical standards: the study was approved by the Ethics Committee of St. Luka LSMU, Ministry of Health of the Russian Federation (Minutes No. 1 of January 27, 2026), conducted in accordance with the Recommendations of the Board of the Eurasian Economic Commission of November 14, 2023 No. 33 "Guidelines for working with laboratory (experimental) animals during preclinical (non-clinical) studies," in compliance with the principles of the European Convention for the Protection of Vertebrates used for Experimental and Other Scientific Purposes, as well as in accordance with the Directive of the
Received: 2026-05-15
Accepted: 2026-06-16
Published online: 2026-06-30
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Fig. 1. Dynamics of changes in the total number of bone marrow cells (×106/ml) in experimental animals of various groups on the 3rd day of the study. In group 5, the absolute value of cellularity corresponded to 1 × 105 cells/ml, which is equivalent to 0.1 × 106 cells/ml on the graph. The data is presented as M±SD. The statistical analysis was performed using one-way ANOVA with Tukey post-hoc test. The differences were considered significant at p < 0.05.
Fig. 2. Dynamics of changes in the population of LSK cells (%) in the bone marrow of experimental animals of various groups on the 3rd day of the study. The data is
presented as M±SD. One-way ANOVA with a Tukey post-hoc test enabled the assessment (p < 0.05)
Table 1. Bone marrow cellularity and population of undifferentiated cells (LSK) in experimental animals of various groups (M ± SD)
Note: * — p < 0.05 compared with the control group; # — p < 0.05 compared with group 2; + — p < 0.05 compared with group 3. In the groups treated with diclofenac sodium at a dose of 3 mg/kg, the cellular index was significantly lower, and therefore the results are presented as ×105 cells/ml.
Table 2. Cytokine concentrations in the bone marrow of experimental animals (pg/ml, M ± SD)
Note: * — p < 0.05 compared with the control group; # — p < 0.05 compared with group 2; + — p < 0.05 compared with group 3.
