ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2017 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
METHOD
Tissue chemiluminescence as a method of evaluation of superoxide radical producing ability of mitochondria
About authors
Department of Medical Biophysics, Faculty of Fundamental Medicine,Lomonosov Moscow State University, Moscow, Russia
Correspondence should be addressed: Aishat Dzhatdoeva
Lomonosovskiy prospekt, d. 31, korp. 5, Moscow, Russia, 119192; moc.liamg@jdtahsya
About paper
Funding: this work was supported by the Russian Science Foundation (project no. 14-15-00375).
Acknowledgments: authors thank Aleksey Grishin, a student at the Department of Physiology, Lomonosov Moscow State University, for his help in choosing gas mixture components.
Received: 2015-10-01
Accepted: 2015-12-09
Published online: 2017-01-05
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Fig. 1. (A) Experimental setup scheme. 1, 2 — tanks for two different gas mixtures (oxygen and oxygen-free mixtures); 3 — gas mixture switch; 4 — tubes supplying gas mixture to the chemiluminometer cuvette; 5 — peristaltic pump feeding gas mixture to the system; 6 — SmartLum-100 chemiluminometer; 7 — computer. (B) Position of the test sample in the chemiluminometer
Fig. 2. (A) Change in pH and (B) development of lucigenin-enhanced chemiluminescence in one of the rat heart samples with aeration of samples with gas mixtures of different composition
1 — curve for exhaled air (O2 — 5 %, CO2 — 4,3 %, N2 — 74,0 %); 2 — curve for atmospheric air (O2 — 21,0 %, CO2 — 0,03 %, N2 — 78,0 %); 3 — curve for carbogen (O2 — 95,0 %, CO2 — 5,0 %). Arrow indicates start of aeration.
Fig. 3. Curves of lucigenin-enhanced chemiluminescence in one of the rat heart samples for different hypoxia models. (A) Model 1 (hypoxia cycle — 15 min). (B) Model 2 (hypoxia cycle - 150 min). (C) Model 3 (hypoxia cycle — 240 min)
Fig. 4. Development of lucigenin-enhanced chemiluminescence in mouse brain tissue sections containing the striatum (A) and substantia nigra (B)
1 — curve for control sample, 2 — curve for test sample.
Table 1. Influence of hypoxia duration on formation of superoxide anion radical in rat heart tissue, M ± m (n = 5, p < 0.05)
Table 2. Formation of superoxide anion radical in mouse brain tissue when modeling parkinsonism, M ± m (n = 5, * — p < 0.05)
