2017 / 03

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DOI: 10.24075/brsmu.2017-03-03

ORIGINAL RESEARCH

Distribution of intravenously injected small interfering RNAs in organs and tissues

Kuzevanova AYu1, Luneva AS2, Maslov MA2, Karpukhin AV1, Alimov AA
About authors

1 Laboratory of Molecular Genetics of Complexly Inherited Diseases,
Research Centre of Medical Genetics, Moscow, Russia

2 Preobrazhensky Department of Chemistry and Technology of Bioactive Compounds,
Institute of Fine Chemical Technologies, Moscow, Russia

Correspondence should be addressed: Andrei Alimov
ul. Moskvorechie, d. 1, Moscow, Russia, 115478; ur.xednay@0102vomila.ierdna

About paper

Acknowledgements: authors thank Olga Konyaeva and Natalia Kulbachevskaya of Blokhin Russian Cancer Research Center for providing consultations and technical assistance with the animals.

Contribution of the authors to this work: Kuzevanova AYu — analysis of literature, data collection, analysis, and interpretation; Luneva AS — preparation of liposomes; Maslov MA — research planning, preparation of liposomes; Karpukhin AV — analysis of literature, research planning, data interpretation, drafting of a manuscript; Alimov AA — analysis of literature, research planning, data collection, analysis and interpretation, drafting of a manuscript. All authors participated in editing of the manuscript.

Received: 2017-06-20 Accepted: 2017-06-24 Published online: 2017-07-19
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  1. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 2001 May 24; 411 (6836): 494–8.
  2. Song E, Lee SK, Wang J, Ince N, Ouyang N, Min J, et al. RNA interference targeting Fas protects mice from fulminant hepatitis. Nat Med. 2003 Mar; 9 (3):347–51.
  3. Zimmermann TS, Lee AC, Akinc A, Bramlage B, Bumcrot D, Fedoruk MN, et al. RNAi-mediated gene silencing in non-human primates. Nature. 2006 May 4. 441 (7089): 111–4.
  4. Coelho T, Adams D, Silva A, Lozeron P, Hawkins PN, Mant T, et al. Safety and efficacy of RNAi therapy for transthyretin amyloidosis. N Engl J Med. 2013 Aug 29; 369 (9): 819–29.
  5. Tabernero J, Shapiro GI, LoRusso PM, Cervantes A, Schwartz GK, Weiss GJ, et al. First-in-humans trial of an RNA interference therapeutic targeting VEGF and KSP in cancer patients with liver involvement. Cancer Discov. 2013 Apr; 3 (4): 406–17.
  6. Geisbert TW, Lee AC, Robbins M, Geisbert JB, Honko AN, Sood V, et al. Postexposure protection of non-human primates against a lethal Ebola virus challenge with RNA interference: a proof-of-concept study. Lancet. 2010 May 29; 375 (9729): 1896–905.
  7. Tracy M. Progress in the Development of LNP Delivery for siRNA Advancing LNPs to the Clinic. In: International Liposome Research Days Meeting; 2010 Aug; Vancouver, Canada;. 2010. p. 1–52.
  8. Wittrup A, Lieberman J. Knocking down disease: a progress report on siRNA therapeutics. Nat Rev Genet. 2015 Sep; 16 (9): 543–52.
  9. Morille M, Passirani C, Vonarbourg A, Clavreul A, Benoit J-P. Progress in developing cationic vectors for non-viral systemic gene therapy against cancer. Biomaterials. 2008 Aug–Sep; 29 (24–25): 3477–96.
  10. Maslov MA, Kabilova TO, Petukhov IA, Morozova NG, Serebrennikova GA, Vlassov VV, et al. Novel cholesterol spermine conjugates provide efficient cellular delivery of plasmid DNA and small interfering RNA. J Controlled Release. 2012 Jun 10; 160 (2): 182–93.
  11. Eguchi A, De Mollerat Du Jeu X, Johnson CD, Nektaria A, Feldstein AE. Liver Bid suppression for treatment of fibrosis associated with non-alcoholic steatohepatitis. J Hepatol. 2016 Mar; 64 (3): 699–707.
  12. Bavykin AS, Korotaeva AA, Poyarkov SV, Syrtsev AV, Tjulandin SA, Karpukhin AV. Double siRNA-targeting of cIAP2 and LIVIN results in synergetic sensitization of HCT-116 cells to oxaliplatin treatment. Onco Targets Ther. 2013 Sep 23; 6: 1333–40.
  13. Liu WL, Stevenson M, Seymour LW, Fisher KD. Quantification of siRNA using competitive qPCR. Nucleic Acids Res. 2009 Jan; 37 (1): e4.
  14. Wong L, Lee K, Russell I, Chen C. Application Notes & Tutorials: Endogenous Controls for Real-Time Quantitation of miRNA Using TaqMan® MicroRNA Assays [Internet]. Version: 28 Aug 2010 [cited 2017 Jun 23]. 8 p. Available from: http://tools.thermofisher.com/content/sfs/brochures/cms_044972.pdf
  15. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. Nature Protoc. 2008; 3 (6): 1101–8.