ORIGINAL RESEARCH

Detecting reactive oxygen species in biological fluids by platinum nanoelectrode applying amperometric method

Vaneev AN1, Alova AV1, Erofeev AS1,2, Gorelkin PV3, Aleksashkin AD1, Beznos OV4, Chesnokova NB4, Kost OA1, Majouga AM1,2,5, Korchev Y6,7, Klyachko NL1
About authors

1 Lomonosov Moscow State University, Moscow

2 National University of Science and Technology "MISiS", Moscow

3 Medical Nanotechnology LLC, Moscow

4 Helmholtz Institute of Ophthalmology, Moscow

5 Mendeleyev University of Chemical Technology of Russia, Moscow

6 Department of Medicine, Imperial College London, London, United Kingdom

7 WPI Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kanazawa, Japan

Correspondence should be addressed: Alexander N. Vaneev
Leninskie gory, 1 bl. 11B, Moscow, 119991; moc.liamg@rdnaskela.veenav

About paper

Funding: the study was supported by the Ministry of Education and Science of the Russian Federation in the context of the Agreement # 14.575.21.0147 (project ID RFMEFI57517X0147).

Received: 2018-06-28 Accepted: 2018-07-25 Published online: 2018-09-28
|

Reactive oxygen species (ROS) are vital metabolites in numerous biological functions. Disorders of cellular mechanisms can cause overproduction of ROS and, subsequently, oxidative damage to DNA, proteins, cells and tissues, which is associated with the pathogenesis of a number of neurodegenerative and inflammatory diseases. Development of highly sensitive, relatively simple and fast-to-implement innovative methods to detect oxidative stress requires understanding of how such disorders relate to the level of ROS. This research aimed to apply the biological fluids' ROS detection method we have developed (using the stable platinum nanoelectrode that allows assessing the level of hydrogen peroxide (H2O2) down to 1 μM) and determine the level of H2O2 in lacrimal and intraocular fluids of rabbits, as well as to investigate how the level of H2O2 changes under the influence of antioxidant therapy. The effect superoxide dismutase (SOD) nanoparticles produce on biological fluids' ROS level was shown. The level of H2O2 in lacrimal fluid increased 10 and 30 min after instillation of SOD nanoparticles. As for the intraocular fluid, H2O2 concentration starts to grow only 30 min after instillation of SOD nanoparticles, which suggests that the they penetrate the internal structures of the eye gradually. The method seems to be of value in the context of eye diseases diagnosing and treatment.

Keywords: antioxidant activity, oxidative stress, platinum nanoelectrode, reactive oxygen species, nanosensor, superoxide dismutase nanoparticles

КОММЕНТАРИИ (0)