2018 / 06

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DOI: 10.24075/brsmu.2018.045

ORIGINAL RESEARCH

Detecting reactive oxygen species in biological fluids by platinum nanoelectrode applying amperometric method

Vaneev AN1, Alova AV1, Erofeev AS1,2, Gorelkin PV3, Aleksashkin AD1, Beznos OV4, Chesnokova NB4, Kost OA1, Majouga AM1,2,5, Korchev Y6,7, Klyachko NL1
About authors

1 Lomonosov Moscow State University, Moscow

2 National University of Science and Technology "MISiS", Moscow

3 Medical Nanotechnology LLC, Moscow

4 Helmholtz Institute of Ophthalmology, Moscow

5 Mendeleyev University of Chemical Technology of Russia, Moscow

6 Department of Medicine, Imperial College London, London, United Kingdom

7 WPI Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kanazawa, Japan

Correspondence should be addressed: Alexander N. Vaneev
Leninskie gory, 1 bl. 11B, Moscow, 119991; moc.liamg@rdnaskela.veenav

About paper

Funding: the study was supported by the Ministry of Education and Science of the Russian Federation in the context of the Agreement # 14.575.21.0147 (project ID RFMEFI57517X0147).

Received: 2018-06-28 Accepted: 2018-07-25 Published online: 2018-09-28
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Fig. 1. Platinum electrodes production pattern
Fig. 2. А. Volt-ampere characteristics for a nanoelectrode against Ag / AgCl (1 V/s). Cyclic volt-amper-grams measured in a 1 mM solution of ferrocene methanol. Black shows the current-voltage characteristic for a carbon nanoelectrode, red — after deposition of platinum. B. Photograph of the manufactured nanoelectrodes. C. Calibration line of the platinum electrode used to determine the concentration of H2O2 in lacrimal and intraocular fluids
Fig. 3. Kinetics of the ROS changes in rabbit tear samples after instillation with SOD nanoparticles. Averaged data for 5 rabbits with standard errors. Difference between ROS levels in test and control eyes of each animal (lacrimal fluid)
Fig. 4. Kinetics of the ROS changes in rabbit intraocular fluid samples after instillation with SOD nanoparticles. Averaged data for 5 rabbits with standard errors. Difference between ROS levels in test and control eyes of each animal (lacrimal fluid)