Analysis of associations of polymorphisms in the genes coding for L4, IL10, IL13 with the development of atopic bronchial asthma and its remission

Zhorina YuV1, Abramovskikh OS1, Ignatova GL1, Ploshchanskay OG2
About authors

1 South Ural State Medical University, Chelyabinsk, Russia

2 DNA Clinic LLC, Chelyabinsk, Russia

Correspondence should be addressed: Yulia Yu. Zhorina
Vorovskogo, 64, Chelyabinsk, 454092; ur.liam@42tramailuj

About paper

Author contribution: Zhorina YuV — conceived and planned the study, collected, processed and interpreted the data, performed statistical analysis, participated in writing the manuscript; Abramovskikh OS — proposed the method and supervised the study, analyzed and interpreted the data, participated in writing the manuscript; Ignatova GL — analyzed the clinical data, supervised the study, interpreted the data, participated in writing the manuscript; Ploshchanskay OG — collected the data, conducted laboratory tests, interpreted the data and participated in writing the manuscript.

Received: 2019-09-25 Accepted: 2019-10-11 Published online: 2019-10-22

Bronchial asthma is a multifactorial disease underpinned by chronic inflammation. The atopic phenotype of BA implies the presence of similar molecular mechanisms of pathogenesis between the patients. The aim of this study was to analyze the associations between the development of atopic BA/its remission and the following polymorphisms of interleukin genes: IL4 (rs2243250; C-589T), IL10 (rs1800896; G-1082A; rs1800872; C-592A), and IL13 (rs20541; Arg130Gln). Using allele-specific polymerase chain reaction (PCR), we studied the listed SNPs in the mixed urban sample of patients with BA (n = 53) and the controls (n = 30) residing in South Ural. The analysis revealed that genotype АА of IL10 (rs1800872) occurred more frequently in the control group (23.3%) than in the patients with atopic BA (5.7%) (OR = 0.197; 95% CI [0.047–0.832]; р = 0.031). No differences in genotype frequencies were observed between the patients with atopic BA and the controls for other studied polymorphisms. Our study failed to demonstrate the association of the listed polymorphisms and BA remission.

Keywords: cytokines, gene polymorphism, bronchial asthma in adults, atopy, remission