OPINION

Autonomous bioluminescent systems: prospects for use in the imaging of living organisms

About authors

1 Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia

2 Pirogov Russian National Research Medical University, Moscow, Russia

Correspondence should be addressed: Aleksandr S. Shcheglov
Miklukho-Маklaya, 16/10, Moscow, 117997; ur.liam@trakuj

About paper

Funding: the study was supported by the Russian Science Foundation (Grant №17-14-01169).

Acknowledgements: we thank to the Center for Precision Genome Editing and Genetic Technologies for Biomedicine (Moscow) for the genetic research methods.

Author contribution: Osipova ZM, Shcheglov AS — literature analysis, article authoring; Yampolsky IV — study planning, manuscript editing.

Received: 2019-12-03 Accepted: 2019-12-12 Published online: 2019-12-20
|

Bioluminescent systems are increasingly being used for the development of highly sensitive optical imaging techniques in vivo. However, it is necessary to inject expensive and unstable synthetic substrates (luciferins) before each analysis for most of the systems applied. Autonomous bacterial and fungal bioluminescent systems, that recently have become available for implementation in eukaryotic cells, in our opinion, may be developed into an effective tool in new technologies of bioluminescent imaging.

Keywords: bioluminescence, bioimaging, luciferin, luciferase, biomedical research, autonomous bioluminescence

КОММЕНТАРИИ (0)