ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
A mutant of the phototoxic protein KillerRed that does not form DsRed-like chromophore
About authors
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia
Correspondence should be addressed: Karen S. Sarkisyan
Miklukho-Maklaya, 16/10, of. 34/632, Moscow, 117997; moc.liamg@naysikras.s.nerak
About paper
Funding: this work would not have been published without the generous support and the strict publication requirements of the Russian Foundation for Basic Research grant 18-04-01173. KSS is supported by the president fellowship 075-15-2019-411. Experiments were partially carried out using the equipment provided by the Institute of Bioorganic Chemistry of the Russian Academy of Sciences Сore Facility (CKP IBCH; supported by Russian Ministry of Education and Science Grant RFMEFI62117X0018).
Acknowledgements: we thank to the Center for Precision Genome Editing and Genetic. Technologies for Biomedicine (Moscow) for the genetic research methods.
Author contribution: Sarkisyan KS, Gorbachev DA — conceived and planned the project, performed the experiments, analysed data and prepared the manuscript.
Received: 2019-12-08
Accepted: 2019-12-18
Published online: 2019-12-20
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Fig. 1. Absorption spectra of purified proteins KillerRed and KillerRed I64L/D114G/T115S
Fig. 2. Location of the chromophore and mutations I64L, D114G and T115S in the structure of KillerRed.
Table. Amino acid sequences of proteins described in this study. Chromophore-forming residues are highlighted in purple, positions containing mutations are highlighted
in orange
