ORIGINAL RESEARCH

The role of some xenobiotic biotransformation genes SNP in the development of acute pancreatitis

Samgina TA, Nazarenko PM, Polonikov AV, Lazarenko VA
About authors

Kursk State Medical University, Kursk, Russia

Correspondence should be addressed: Tatiana A. Samgina
K. Marksa, 3, 305000; Kursk, ur.tsil@ssat

About paper

Author contribution: Samgina TA conceived and designed the study, conducted clinical and molecular-genetic tests, analyzed and interpreted the obtained data, and wrote the manuscript; Nazarenko PM supervised surgical treatment and postoperative care at Kursk City Clinical Hospital № 4 and recruited patients for the study; Polonikov AV supervised genetic testing; Lazarenko VA supervised the study.

Received: 2020-01-05 Accepted: 2020-02-08 Published online: 2020-02-16
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Genetically determined features of the xenobiotic biotransformation system play an important role in the development of acute pancreatitis (AP) and its complications. The aim of this study was to assess the contribution of 3 SNPs (CYP1A1 -462 T>C rs1048943, CYP2E1 -1293 G>C rs3813867 and ABCB1 -3435 G>A rs1045642) to the development of AP and its complications. DNA samples were collected from 547 unrelated patients with AP (154 women and 393 men; mean age 48.9 ± 13.1 years) undergoing therapy at surgery departments of Kursk and 573 unrelated individuals without gastrointestinal diseases (161 women and 412 men; mean age 47.8 ± 12.1 years). The polymorphisms were genotyped by PCR using TaqMan probes for allele discrimination. Infected pancreatic necrosis (IPN) was observed in 97 patients; 101 patients developed a pseudocyst (PC); 111 patients had a peripancreatic necrosis (PN). AP was the most common in the carriers of the А allele in ABCB1 G>A (rs1045642) (p = 0.0008). The carriers of the G/G genotype rarely developed both AP (p = 5·10–4) and its complications: IPN (p = 0.03R), PN (p = 0.036R), PC (p = 0.04R). The carriers of the G/C–C/C CYP2E1 G>C (rs3813867) genotypes who had no long-term history of alcohol abuse rarely developed AP (p = 0.03). The carriers of the G/C CYP2E1 (rs3813867) genotype tended to develop pseudocysts (p = 0.05OD). AP was more frequently complicated by IPN (p = 0.009R), PN (p = 0.003R) and PC (p = 0.003D) in the carriers of the C/C CYP1A1 T>C (rs1048943) genotype. A milder course of AP was typical for the carriers of the G/G ABCB1 G>A (rs1045642) genotype; a more severe course was characteristic of the carriers of the C/C CYP1A1 T>C (rs1048943) genotype.

Keywords: acute pancreatitis, genetic polymorphism, xenobiotic biotransformation enzyme genes, rs1045642, rs1048943, rs3813867

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